Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy

被引：2

作者：

Ghassemi, Saba ^{[1
]}

Milone, Michael C. ^{[1
]}

机构：

[1] Univ Penn, Perelman Sch Med, Ctr Cellular Immunotherapies, Philadelphia, PA 19104 USA

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2019年 / 154期

关键词：

Cancer Research; Issue; 154; adoptive immunotherapy; chimeric antigen receptors; CAR T cell manufacturing; cancer; T cell; CAR T cell differentiation; MEMORY; THERAPY; IL-7;

D O I：

10.3791/59949

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Adoptive immunotherapy holds promise for the treatment of cancer and infectious disease. We describe a simple approach to transduce primary human T cells with chimeric antigen receptor (CAR) and expand their progeny ex vivo. We include assays to measure CAR expression as well as differentiation, proliferative capacity and cytolytic activity. We describe assays to measure effector cytokine production and inflammatory cytokine secretion in CAR T cells. Our approach provides a reliable and comprehensive method to culture CAR T cells for preclinical models of adoptive immunotherapy.

引用

页数：6

共 25 条

[1] Adoptive transfer of effector CD8+ T cells derived from central memory cells establishes persistent T cell memory in primates [J].

Berger, Carolina ;

Jensen, Michael C. ;

Lansdorp, Peter M. ;

Gough, Mike ;

Elliott, Carole ;

Riddell, Stanley R. .

JOURNAL OF CLINICAL INVESTIGATION, 2008, 118 (01) :294-305

[2] T-cell therapy in the treatment of post-transplant lymphoproliferative disease [J].

Bollard, Catherine M. ;

Rooney, Cliona M. ;

Heslop, Helen E. .

NATURE REVIEWS CLINICAL ONCOLOGY, 2012, 9 (09) :510-519

[3] CD19-Targeted T Cells Rapidly Induce Molecular Remissions in Adults with Chemotherapy-Refractory Acute Lymphoblastic Leukemia [J].

Brentjens, Renier J. ;

Davila, Marco L. ;

Riviere, Isabelle ;

Park, Jae ;

Wang, Xiuyan ;

Cowell, Lindsay G. ;

Bartido, Shirley ;

Stefanski, Jolanta ;

Taylor, Clare ;

Olszewska, Malgorzata ;

Borquez-Ojeda, Oriana ;

Qu, Jinrong ;

Wasielewska, Teresa ;

He, Qing ;

Bernal, Yvette ;

Rijo, Ivelise V. ;

Hedvat, Cyrus ;

Kobos, Rachel ;

Curran, Kevin ;

Steinherz, Peter ;

Jurcic, Joseph ;

Rosenblat, Todd ;

Maslak, Peter ;

Frattini, Mark ;

Sadelain, Michel .

SCIENCE TRANSLATIONAL MEDICINE, 2013, 5 (177)

[4] Adoptive T-Cell Therapy of a Lung Transplanted Patient with Severe CMV Disease and Resistance to Antiviral Therapy [J].

Brestrich, G. ;

Zwinger, S. ;

Fischer, A. ;

Schmueck, M. ;

Roehmhild, A. ;

Hammer, M. H. ;

Kurtz, A. ;

Uharek, L. ;

Knosalla, C. ;

Lehmkuhl, H. ;

Volk, H. -D. ;

Reinke, P. .

AMERICAN JOURNAL OF TRANSPLANTATION, 2009, 9 (07) :1679-1684

[5] IL-7 and IL-15 instruct the generation of human memory stem T cells from naive precursors [J].

Cieri, Nicoletta ;

Camisa, Barbara ;

Cocchiarella, Fabienne ;

Forcato, Mattia ;

Oliveira, Giacomo ;

Provasi, Elena ;

Bondanza, Attilio ;

Bordignon, Claudio ;

Peccatori, Jacopo ;

Ciceri, Fabio ;

Lupo-Stanghellini, Maria Teresa ;

Mavilio, Fulvio ;

Mondino, Anna ;

Bicciato, Silvio ;

Recchia, Alessandra ;

Bonini, Chiara .

BLOOD, 2013, 121 (04) :573-584

[6] IL-7-Induced Glycerol Transport and TAG Synthesis Promotes Memory CD8+ T Cell Longevity [J].

Cui, Guoliang ;

Staron, Matthew M. ;

Gray, Simon M. ;

Ho, Ping-Chih ;

Amezquita, Robert A. ;

Wu, Jingxia ;

Kaech, Susan M. .

CELL, 2015, 161 (04) :750-761

[7] Determinants of response and resistance to CD19 chimeric antigen receptor (CAR) T cell therapy of chronic lymphocytic leukemia [J].

Fraietta, Joseph A. ;

Lacey, Simon F. ;

Orlando, Elena J. ;

Pruteanu-Malinici, Iulian ;

Gohil, Mercy ;

Lundh, Stefan ;

Boesteanu, Alina C. ;

Wang, Yan ;

O'Connor, Roddy S. ;

Hwang, Wei-Ting ;

Pequignot, Edward ;

Ambrose, David E. ;

Zhang, Changfeng ;

Wilcox, Nicholas ;

Bedoya, Felipe ;

Dorfmeier, Corin ;

Chen, Fang ;

Tian, Lifeng ;

Parakandi, Harit ;

Gupta, Minnal ;

Young, Regina M. ;

Johnson, F. Brad ;

Kulikovskaya, Irina ;

Liu, Li ;

Xu, Jun ;

Kassim, Sadik H. ;

Davis, Megan M. ;

Levine, Bruce L. ;

Frey, Noelle V. ;

Siegel, Donald L. ;

Huang, Alexander C. ;

Wherry, E. John ;

Bitter, Hans ;

Brogdon, Jennifer L. ;

Porter, David L. ;

June, Carl H. ;

Melenhorst, J. Joseph .

NATURE MEDICINE, 2018, 24 (05) :563-+

[8] A human memory T cell subset with stem cell-like properties [J].

Gattinoni, Luca ;

Lugli, Enrico ;

Ji, Yun ;

Pos, Zoltan ;

Paulos, Chrystal M. ;

Quigley, Maire F. ;

Almeida, Jorge R. ;

Gostick, Emma ;

Yu, Zhiya ;

Carpenito, Carmine ;

Wang, Ena ;

Douek, Daniel C. ;

Price, David A. ;

June, Carl H. ;

Marincola, Francesco M. ;

Roederer, Mario ;

Restifo, Nicholas P. .

NATURE MEDICINE, 2011, 17 (10) :1290-U325

[9] Reducing Ex Vivo Culture Improves the Antileukemic Activity of Chimeric Antigen Receptor (CAR) T Cells [J].

Ghassemi, Saba ;

Nunez-Cruz, Selene ;

O'Connor, Roddy S. ;

Fraietta, Joseph A. ;

Patel, Prachi R. ;

Scholler, John ;

Barrett, David M. ;

Lundh, Stefan M. ;

Davis, Megan M. ;

Bedoya, Felipe ;

Zhang, Changfeng ;

Leferovich, John ;

Lacey, Simon F. ;

Levine, Bruce L. ;

Grupp, Stephan A. ;

June, Carl H. ;

Melenhorst, J. Joseph ;

Milone, Michael C. .

CANCER IMMUNOLOGY RESEARCH, 2018, 6 (09) :1100-1109

[10] Chimeric Antigen Receptor-Modified T Cells for Acute Lymphoid Leukemia [J].

Grupp, Stephan A. ;

Kalos, Michael ;

Barrett, David ;

Aplenc, Richard ;

Porter, David L. ;

Rheingold, Susan R. ;

Teachey, David T. ;

Chew, Anne ;

Hauck, Bernd ;

Wright, J. Fraser ;

Milone, Michael C. ;

Levine, Bruce L. ;

June, Carl H. .

NEW ENGLAND JOURNAL OF MEDICINE, 2013, 368 (16) :1509-1518

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