The characterization of a subgenomic RNA and in vitro translation products of oat chlorotic stunt virus

被引:3
作者
Boonham, N [1 ]
Henry, CM
Wood, KR
机构
[1] Cent Sci Lab, York YO4 1LZ, N Yorkshire, England
[2] Univ Birmingham, Sch Biol Sci, Birmingham B15 2TT, W Midlands, England
关键词
OCSV; subgenomic RNA; translation; tombusviridae;
D O I
10.1023/A:1007985404933
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Oat chlorotic stunt virus (OCSV) is a 35 nm icosahedral plant virus comprising of a single capsid protein with a Mr of 48.2 kDa and a 4.1 kb single stranded, positive sense genomic RNA. Northern blot analysis detected a single 3' terminal subgenomic RNA in extracts from infected plants, which was also found to be encapsidated. Virion RNA directs the synthesis of a 23 kDa polypeptide in a rabbit reticulocyte in vitro translation system. Primer extension analysis has been used to map the end of both the genomic and subgenomic RNA's, and has shown the genomic size to be 4115 nucleotides in length. The results have enabled a model for the genome expression to be proposed.
引用
收藏
页码:141 / 145
页数:5
相关论文
共 12 条
[1]   THE NUCLEOTIDE-SEQUENCE AND PROPOSED GENOME ORGANIZATION OF OAT CHLOROTIC STUNT VIRUS, A NEW SOIL-BORNE VIRUS OF CEREALS [J].
BOONHAM, N ;
HENRY, CM ;
WOOD, KR .
JOURNAL OF GENERAL VIROLOGY, 1995, 76 :2025-2034
[2]   LINEAR-LOG SUCROSE GRADIENTS FOR ESTIMATING SEDIMENTATION COEFFICIENTS OF PLANT VIRUSES AND NUCLEIC ACIDS [J].
BRAKKE, MK ;
VANPELT, N .
ANALYTICAL BIOCHEMISTRY, 1970, 38 (01) :56-&
[3]   REPLICATION AND MOVEMENT OF A COAT PROTEIN MUTANT OF CYMBIDIUM RINGSPOT TOMBUSVIRUS [J].
DALMAY, T ;
RUBINO, L ;
BURGYAN, J ;
RUSSO, M .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1992, 5 (05) :379-383
[4]   INTERCISTRONIC AS WELL AS TERMINAL SEQUENCES ARE REQUIRED FOR EFFICIENT AMPLIFICATION OF BROME MOSAIC-VIRUS RNA3 [J].
FRENCH, R ;
AHLQUIST, P .
JOURNAL OF VIROLOGY, 1987, 61 (05) :1457-1465
[5]   TURNIP CRINKLE VIRUS GENES REQUIRED FOR RNA REPLICATION AND VIRUS MOVEMENT [J].
HACKER, DL ;
PETTY, ITD ;
WEI, N ;
MORRIS, TJ .
VIROLOGY, 1992, 186 (01) :1-8
[6]   POINT MUTATIONS IN THE TURNIP CRINKLE VIRUS CAPSID PROTEIN AFFECT THE SYMPTOMS EXPRESSED BY NICOTIANA-BENTHAMIANA [J].
HEATON, LA ;
LEE, TC ;
WEI, N ;
MORRIS, TJ .
VIROLOGY, 1991, 183 (01) :143-150
[7]   IMPROVED METHOD FOR THE ISOLATION OF RNA FROM PLANT-TISSUES [J].
LOGEMANN, J ;
SCHELL, J ;
WILLMITZER, L .
ANALYTICAL BIOCHEMISTRY, 1987, 163 (01) :16-20
[8]   IDENTIFICATION OF THE MAIZE CHLOROTIC MOTTLE VIRUS CAPSID PROTEIN CISTRON AND CHARACTERIZATION OF ITS SUBGENOMIC MESSENGER-RNA [J].
LOMMEL, SA ;
KENDALL, TL ;
XIONG, Z ;
NUTTER, RC .
VIROLOGY, 1991, 181 (01) :382-385
[9]  
LUTKE HA, 1987, EMBO J, V6, P43
[10]  
Matthews R.E.F., 1991, PLANT VIROLOGY