Spatial alignment of 3D printed scaffolds modulates genotypic expression in pre-osteoblasts

被引：8
作者：
Nagiah, Naveen [1 ,2 ,3 ]
Bhattacharjee, Maumita [1 ,2 ,3 ]
Murdock, Christopher J. [1 ,2 ]
Kan, Ho-Man [1 ,2 ,3 ]
Barajaa, Mohammed [1 ]
Laurencin, Cato T. [1 ,2 ,3 ,4 ,5 ]
机构：
[1] Univ Connecticut Hlth, Connecticut Convergence Inst Translat Regenerat E, 263 Farmington Ave, Farmington, CT 06030 USA
[2] Univ Connecticut Hlth, Raymond & Beverly Sackler Ctr Biomed Biol Phys &, Farmington, CT USA
[3] Univ Connecticut Hlth, Dept Orthopaed Surg, Farmington, CT USA
[4] Univ Connecticut, Dept Biomed Engn, Storrs, CT USA
[5] Univ Connecticut, Dept Mat Sci & Engn, Storrs, CT USA
来源：
MATERIALS LETTERS | 2020年 / 276卷
关键词：
3D printing; Gelatin; Sodium alginate; Gene expression; Pore geometry; BONE; CARTILAGE; MATRICES;
D O I：
10.1016/j.matlet.2020.128189
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
3D printing, an advent from rapid prototyping technology is emerging as a suitable solution for various regenerative engineering applications. In this study, blended gelatin-sodium alginate 3D printed scaffolds with different pore geometries were developed by altering the spatial alignment of even layered struts in the scaffolds. A significant difference in compression modulus and osteogenic expression due to the difference in spatial printing was demonstrated. Pore geometry was found to be more dominant than the compressive modulus of the scaffold in regulating osteogenic gene expression. A shift in pore geometry by at least 45 degrees was critical for significant increase in osteogenic gene expression in MC3T3-E1 cells. (C) 2020 Elsevier B.V. All rights reserved.
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页数：4
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