Analyses of compound libraries obtained by high-throughput parallel synthesis:: strategy of quality control by high-performance liquid chromatography, mass spectrometry and nuclear magnetic resonance techniques

被引：27
作者：
Duléry, BD
Verne-Mismer, J
Wolf, E
Kugel, C
Van Hijfte, L
机构：
[1] Synthelabo Biomol, Controle Qual Analyt, F-67080 Strasbourg, France
[2] Synthelabo Rech, F-91385 Chilly Mazarin, France
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1999年 / 725卷 / 01期
关键词：
parallel synthesis; compound libraries;
D O I：
10.1016/S0378-4347(98)00570-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The growing interest in combinatorial chemistry has led to a new source of compounds from which a large number of leads has emerged over recent years. Parallel synthesis, in particular, allows a quick production of a wide number of individual compounds. A rapid analytical control is needed to determine their quality. A strategy using automated, fast reversed-phase C18 high-performance liquid chromatography with diode-array detection (LC-DAD-MS) followed by atmospheric pressure chemical ionisation mass spectrometry (APCI-MS) and NMR has been developed for their characterisation and purity control. Complementary NMR analyses are done on selected compounds to provide a better structural characterisation of the expected compounds and their potential side-products. Validated libraries are then registered in ISIS databases using automated procedures. (C) 1999 Elsevier Science B.V. All rights reserved.
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收藏
页码：39 / 47
页数：9
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