Electrophoretic confirmation of sperm mixing in mated Colorado potato beetles (Coleoptera: Chrysomelidae)

被引：10

作者：

Alyokhin, AV ^{[1
]}

Ferro, DN ^{[1
]}

机构：

[1] Univ Massachusetts, Dept Entomol, Amherst, MA 01003 USA

来源：

ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA | 1999年 / 92卷 / 02期

关键词：

Leptinotarsa decemlineata; sperm precedence; allozymes; cellulose acetate gel electrophoresis; phosphoglucomutase;

D O I：

10.1093/aesa/92.2.230

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Sperm precedence in the Colorado potato beetle, Leptinotarsa decemlineata (Say), was investigated using phosphoglucomutase (Pgm) allozymes as genetic markers. Females were mated to 2 males carrying different Pgm alleles, and the proportion of offspring sired by each male was determined by cellulose acetate gel electrophoresis. Our results indicate that Pgm alleles in the Colorado potato beetle are inherited in the Mendelian fashion, and there is no change in sperm utilization over time. We also were able to confirm that sperm precedence in this insect is incomplete, with approximate to 72% of the larvae fathered by males at the 2nd mating. Possible implications of incomplete sperm precedence for insecticide resistance management are discussed.

引用

页码：230 / 235

页数：6

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