Maskless localized patterning of biomolecules on carbon nanotube microarray functionalized by ultrafine atmospheric pressure plasma jet using biotin-avidin system

被引：12
作者：
Abuzairi, Tomy [1 ,2 ]
Okada, Mitsuru [3 ]
Purnamaningsih, Retno Wigajatri [2 ]
Poespawati, Nji Raden [2 ]
Iwata, Futoshi [1 ,3 ,4 ]
Nagatsu, Masaaki [1 ,3 ,4 ]
机构：
[1] Shizuoka Univ, Grad Sch Sci & Technol, Naka Ku, 3-5-1 Johoku, Hamamatsu, Shizuoka 4328561, Japan
[2] Univ Indonesia, Dept Elect Engn, Fac Engn, Depok 16424, Indonesia
[3] Shizuoka Univ, Grad Sch Integrated Sci & Technol, Naka Ku, 3-5-1 Johoku, Hamamatsu, Shizuoka 4328561, Japan
[4] Shizuoka Univ, Elect Res Inst, Naka Ku, 3-5-1 Johoku, Hamamatsu, Shizuoka 4328011, Japan
来源：
APPLIED PHYSICS LETTERS | 2016年 / 109卷 / 02期
基金：
日本学术振兴会;
关键词：
DNA MICROARRAYS; OPTIMIZATION; FABRICATION;
D O I：
10.1063/1.4958988
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
Ultrafine plasma jet is a promising technology with great potential for nano-or micro-scale surface modification. In this letter, we demonstrated the use of ultrafine atmospheric pressure plasma jet (APPJ) for patterning bio-immobilization on vertically aligned carbon nanotube (CNT) microarray platform without a physical mask. The biotin-avidin system was utilized to demonstrate localized bio-molecule patterning on the biosensor devices. Using +/- 7.5 kV square-wave pulses, the optimum condition of plasma jet with He/NH3 gas mixture and 2.5 s treatment period has been obtained to functionalize CNTs. The functionalized CNTs were covalently linked to biotin, bovine serum albumin (BSA), and avidin-(fluorescein isothiocyanate) FITC, sequentially. BSA was necessary as a blocking agent to protect the untreated CNTs from avidin adsorption. The localized patterning results have been evaluated from avidin-FITC fluorescence signals analyzed using a fluorescence microscope. The patterning of biomolecules on the CNT microarray platform using ultrafine APPJ provides a means for potential application of microarray biosensors based on CNTs. Published by AIP Publishing.
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页数：4
相关论文
共 30 条
[1]   Mask less functionalization of a carbon nanotube dot array biosensor using an ultrafine atmospheric pressure plasma jet [J].
Abuzairi, Tomy ;
Okada, Mitsuru ;
Mochizuki, Yohei ;
Poespawati, Nji Raden ;
Purnamaningsih, Retno Wigajatri ;
Nagatsu, Masaaki .
CARBON, 2015, 89 :208-216
[2]   Bio-microarray fabrication techniques - A review [J].
Barbulovic-Nad, Irena ;
Lucente, Michael ;
Sun, Yu ;
Zhang, Mingjun ;
Wheeler, Aaron R. ;
Bussmann, Markus .
CRITICAL REVIEWS IN BIOTECHNOLOGY, 2006, 26 (04) :237-259
[3]   Adsorption of avidin on microfabricated surfaces for protein biochip applications [J].
Bashir, R ;
Gomez, R ;
Sarikaya, A ;
Ladisch, MR ;
Sturgis, J ;
Robinson, JP .
BIOTECHNOLOGY AND BIOENGINEERING, 2001, 73 (04) :324-328
[4]   Trehalose glycopolymer resists allow direct writing of protein patterns by electron-beam lithography [J].
Bat, Erhan ;
Lee, Juneyoung ;
Lau, Uland Y. ;
Maynard, Heather D. .
NATURE COMMUNICATIONS, 2015, 6
[5]   Fabrication of biological microarrays using microcantilevers [J].
Belaubre, P ;
Guirardel, M ;
Garcia, G ;
Pourciel, JB ;
Leberre, V ;
Dagkessamanskaia, A ;
Trévisiol, E ;
François, JM ;
Bergaud, C .
APPLIED PHYSICS LETTERS, 2003, 82 (18) :3122-3124
[6]   Optimisation of a silicon/silicon dioxide substrate for a fluorescence DNA microarray [J].
Bras, M ;
Dugas, V ;
Bessueille, F ;
Cloarec, JP ;
Martin, JR ;
Cabrera, M ;
Chauvet, JP ;
Souteyard, E ;
Garrigues, M .
BIOSENSORS & BIOELECTRONICS, 2004, 20 (04) :797-806
[7]   Pseudo 3D single-walled carbon nanotube film for BSA-free protein chips [J].
Byon, HR ;
Hong, BJ ;
Gho, YS ;
Park, JW ;
Choi, HC .
CHEMBIOCHEM, 2005, 6 (08) :1331-1334
[8]   Self-aligned patterns of multiple biomolecules printed in one step [J].
Chalmeau, J. ;
Thibault, C. ;
Carcenac, F. ;
Vieu, C. .
APPLIED PHYSICS LETTERS, 2008, 93 (13)
[9]   Amino group introduction onto multiwall carbon nanotubes by NH3/Ar plasma treatment [J].
Chen, Changlun ;
Liang, Bo ;
Lu, Di ;
Ogino, Akihisa ;
Wang, Xiangke ;
Nagatsu, Masaaki .
CARBON, 2010, 48 (04) :939-948
[10]   Protein microarrays with carbon nanotubes as multicolor Raman labels [J].
Chen, Zhuo ;
Tabakman, Scott M. ;
Goodwin, Andrew P. ;
Kattah, Michael G. ;
Daranciang, Dan ;
Wang, Xinran ;
Zhang, Guangyu ;
Li, Xiaolin ;
Liu, Zhuang ;
Utz, Paul J. ;
Jiang, Kaili ;
Fan, Shoushan ;
Dai, Hongjie .
NATURE BIOTECHNOLOGY, 2008, 26 (11) :1285-1292
123