Effects of relaxin on vascular smooth muscle and endothelial cells in normotensive and hypertensive rats

被引：14

作者：

Failli, P

Nistri, S

Mazzetti, L

Chiappini, L

Bani, D

机构：

[1] Univ Florence, Dept Anat Histol & Forens Med, I-50139 Florence, Italy

[2] Univ Florence, Dept Preclin & Clin Pharmacol, I-50139 Florence, Italy

来源：

RELAXIN AND RELATED PEPTIDES: FOURTH INTERNATIONAL CONFERENCE | 2005年 / 1041卷

关键词：

endothelial cells; smooth muscle cells; SHR; calcium; nitric oxide; protein kinase G;

D O I：

10.1196/annals.1282.048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We tested the effects of relaxin on [Ca2+](i) response to angiotensin II in smooth muscle (vSMC) and endothelial cells isolated from hypertensive (SHR) and normotensive (WKY) rats. Relaxin markedly reduced the [Ca2+](i) response of vSMCs from WKY, but not from SHR rats. Western blots showed that cGMP-dependent protein kinase G was reduced in vSMCs from SHR as compared with WKY rats. Relaxin also blunted the [Ca2+] i response in endothelial cells from WKY, but not from SHR rats. However, in endothelial cells from SHR and WKY rats, protein kinase G was nearly unexpressed, thus accounting for an alternative pathway of the intracellular response to nitric oxide and relaxin. Hence, vSMCs and endothelial cells in SHR rats show a deficiency response to nitric oxide that may render them insensitive to relaxin.

引用

页码：311 / 313

页数：3

共 6 条

[1] Relaxin activates the L-arginine nitric oxide pathway in vascular smooth muscle cells in culture [J].