The streptozotocin-induced rat model of diabetes mellitus evidences significant reduction of myosin-Va expression in the brain

被引：10

作者：

da Costa, Alice Vieira ^{[1
]}

Calabria, Luciana Karen ^{[1
]}

Nascimento, Rafael ^{[1
]}

Carvalho, Washington Joao ^{[1
]}

Goulart, Luiz Ricardo ^{[1
]}

Espindola, Foued Salmen ^{[1
]}

机构：

[1] Univ Fed Uberlandia, Inst Genet & Biochem, BR-38400902 Uberlandia, MG, Brazil

来源：

METABOLIC BRAIN DISEASE | 2011年 / 26卷 / 04期

关键词：

Diabetes mellitus; Streptozotocin; Brain; Myosin-Va; GRISCELLI-SYNDROME; NERVOUS-SYSTEM; LOCALIZATION; CELLS; HIPPOCAMPUS; PLASTICITY; PROTEINS; DISEASE; BINDING;

D O I：

10.1007/s11011-011-9259-5

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Diabetes mellitus is a disease characterized by increased glucose levels in the blood. Hyperglycemia causes damage to the brain tissue, and induces significant changes in synaptic transmission. In this investigation, we have found a significant alteration in the expression of the molecular motor involved in the synaptic vesicles transport, myosin-Va, and its distribution in rat brains of streptozotocin-induced diabetes model. Brains were removed after 20 days, homogenized and analysed by Western blotting, qRT-PCR and immunohistochemistry. Myosin-Va presented significantly lower levels of both mRNA and protein in diabetic than those observed in non-diabetic animals. Moreover, neuronal and glial cells of the occipital and frontal cortex exhibited decreased myosin-Va immunostaining in diabetic rat brains. In conclusion, diabetic rat brains displayed altered expression and distribution of myosin-Va, and these finding may contribute to the basic understanding about this myosin role in brain function related to diabetes.

引用

页码：247 / 251

页数：5

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