Biosynthesis, characterization, bactericidal and sporicidal activity of silver nanoparticles using the leaves extract of Litchi chinensis

Biosynthesis of silver nanoparticles (AgNPs) using plant extracts has become a promising alternative to the conventional chemical synthesis approach. In this study, cost-effective synthesis of AgNPs was attempted using leaves extract of Litchi chinensis. Bio-reduction reaction for the synthesis of NPs was checked by confirming the presence of AgNPs in solution by UV-vis spectrophotometry and with further characterization by fourier-transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). Surface plasmon resonance (SPR) band showed absorption peak at 422 nm indicating the formation of AgNPs, and FTIR spectra confirmed the presence of biological molecules involved in AgNPs synthesis. TEM analysis revealed the spherical shape of AgNPs with particle size distribution in a range of 5-15 nm. Further, the biosynthesized AgNPs showed significant bactericidal and sporicidal activity against model spore former Bacillus subtilis. AgNPs at concentrations ranging from 25 to 100 mu g/mL showed bactericidal activity with inhibition zone ranging from 4-19 mm and sporicidal activity at 100-200 mu g/mL in a range of 4.46-61.6% with an exposure time of 2-8 h. These findings exhibit distinctive potential of biogenic AgNPs for their efficient use in developing novel bactericidal and sporicidal agent against spore forming bacilli.