Comparative Evaluation of Real-Time PCR Methods for Human Noroviruses in Wastewater and Human Stool

被引：10

作者：

Masago, Yoshifumi ^{[1
,3
]}

Konta, Yoshimitsu ^{[1
]}

Kazama, Shinobu ^{[1
]}

Inaba, Manami ^{[1
]}

Imagawa, Toshifumi ^{[2
,4
]}

Tohma, Kentaro ^{[2
]}

Saito, Mayuko ^{[2
]}

Suzuki, Akira ^{[2
,5
]}

Oshitani, Hitoshi ^{[2
]}

Omura, Tatsuo ^{[1
]}

机构：

[1] Tohoku Univ, New Ind Creat Hatchery Ctr, Sendai, Miyagi, Japan

[2] Tohoku Univ, Grad Sch Med, Sendai, Miyagi, Japan

[3] United Nations Univ, Inst Adv Study Sustainabil, Shibuya Ku, Tokyo, Japan

[4] Natl Inst Infect Dis, Dept Virol 2, Tokyo, Japan

[5] Tohoku Univ, Dept Pediat, Grad Sch Med, Sendai, Miyagi, Japan

来源：

PLOS ONE | 2016年 / 11卷 / 08期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

REVERSE-TRANSCRIPTION-PCR; ENVIRONMENTAL SURVEILLANCE; UNITED-STATES; GENOGROUP-I; RT-PCR; VIRUSES; ASSAYS;

D O I：

10.1371/journal.pone.0160825

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Selecting the best quantitative PCR assay is essential to detect human norovirus genome effectively from clinical and environmental samples because no cell lines have been developed to propagate this virus. The real-time PCR methods for noroviruses GI (4 assays) and GII (3 assays) were evaluated using wastewater (n = 70) and norovirus-positive stool (n = 77) samples collected in Japan between 2012 and 2013. Standard quantitative PCR assays recommended by the U.S. Environmental Protection Agency, International Organization for Standardization, and Ministry of Health, Labour and Welfare, Japan, together with recently reported assays were included. Significant differences in positive rates and quantification cycles were observed by non-parametric analysis. The present study identifies the best assay for norovirus GI and GII to amplify norovirus genomes efficiently.

引用

页数：10

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