A human stool-derived Bilophila wadsworthia strain caused systemic inflammation in specific-pathogen-free mice

被引：143

作者：

Feng, Zhou ^{[1
]}

Long, Wenmin ^{[1
]}

Hao, Binhan ^{[1
]}

Ding, Ding ^{[1
]}

Ma, Xiaoqing ^{[1
]}

Zhao, Liping ^{[1
]}

Pang, Xiaoyan ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, 800 Dongchuan Rd, Shanghai 200240, Peoples R China

来源：

GUT PATHOGENS | 2017年 / 9卷

基金：

中国国家自然科学基金;

关键词：

Bilophila wadsworthia; Inflammation; Hepatosplenomegaly; Gut microbiota; Mice; SERUM AMYLOID-A; GUT MICROBIOTA; AKKERMANSIA-MUCINIPHILA; INSULIN-RESISTANCE; OBESITY; DIET; PCR; ASSOCIATION; ENDOTOXEMIA; INFECTION;

D O I：

10.1186/s13099-017-0208-7

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background: Bilophila wadsworthia is a major member of sulfidogenic bacteria in human gut, it was originally recovered from different clinical specimens of intra-abdominal infections and recently was reported potentially linked to different chronic metabolic disorders. However, there is still insufficient understanding on its detailed function and mechanism to date. Methods: A B. wadsworthia strain was isolated from fresh feces of a latent autoimmune diabetes in adults patient and we investigated its pathogenicity by oral administration to specific-pathogen-free mice. Tissue samples and serum were collected after sacrifice. Stool samples were collected at different time points to profile the gut microbiota. Results: Bilophila wadsworthia infection resulted in the reduction of body weight and fat mass, apparent hepatosplenomegaly and elevated serum inflammatory factors, including serum amyloid A and interleukin-6, while without significant change of the overall gut microbiota structure. Conclusions: These results demonstrated that higher amount of B. wadsworthia caused systemic inflammatory response in SPF mice, which adds new evidence to the pathogenicity of this bacterium and implied its potential role to the chronic inflammation related metabolic diseases like diabetes.

引用

页数：10

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[11] Serum amyloid A links endotoxaemia to weight gain and insulin resistance in mice [J].