Analysis of association of polymorphism 308G>A TNF-α gene with Allergic rhinitis in Uzbekistan

The generalized results of genetic analysis of polymorphism 308 G>A TNF-alpha gene in healthy donors and patients with allergic rhinitis in Uzbekistan were presented in this article. Data on observed frequencies and genotypes of polymorphism 308 G>A TNF-alpha gene was introduced and the evaluation of possible association of this genetic marker with allergic rhinitis was performed. No statistically significant differences were determined in comparative analysis of allele and genotype frequencies of polymorphism 308G>A TNF-alpha gene between general group of patients with AR and control group. The occurrence frequency of polymorphism 308G>A TNF-alpha gene among healthy people was 11.4%, and in control group 7.1% (OR= 1.15;.2= 0.05; p>0.05). However, the results, confirming the presence of association of this marker with intermittent form of AR were obtained during comparative analysis between control group and subgroup of patients, distinguished by the form of AR. The frequency of mutant allele polymorphism 308G>A TNF-alpha gene was 5 times higher in patients with intermittent form of AR than in persistent form of AR (21.1 % against 4.0%;) and more than two times in comparison to controlled group (21.1% against 10.0%; OR= 2.4). Atopy -genetic predisposition to Jg E mediated response to antigens, hyperactivity to physicochemical influence and inflammation, underlying the pathogenesis of this disease is regarded as significant signs of allergic rhinitis (AR). It is commonly known that polymorphism 308G>A TNF-alpha gene is associated essentially with atopic condition and correlated diseases. [1, 2, 3, 10]. TNF-alpha gene factor becomes localized in short chromosome arm 6p21.3 [5, 6]. Despite the obvious clinical and biochemical validity of studies of polymorphism 308G>A TNF-alpha gene in connection with atopic diseases, very few scientific laboratories are devoted to the association of this locus with AR [5-10]. On the basis of the abovementioned fact, there exists the importance of conducting a detailed molecular genetic research of this problem. The purpose of the present work is to observe the frequency of occurrence of alleles and genotypes, as well as possible association of polymorphous marker 308 G>A TNF-alpha gene with the development of AR in Uzbekistan.