Cross-linking/mass spectrometry to get a closer view on protein interaction networks

被引：62

作者：

Iacobucci, Claudio ^{[1
,3
]}

Goetze, Michael ^{[2
,4
]}

Sinz, Andrea ^{[1
]}

机构：

[1] Martin Luther Univ Halle Wittenberg, Charles Tanford Ctr, Dept Pharmaceut Chem & Bioanalyt, Inst Pharm, Kurt Mothes Str 3a, D-06120 Halle, Saale, Germany

[2] Martin Luther Univ Halle Wittenberg, Inst Biochem & Biotechnol, Charles Tanford Prot Ctr, Kurt Mothes Str 3a, D-06120 Halle, Saale, Germany

[3] CHIESI FARMACEUT SpA, Corp Preclin R&D Analyt & Early Formulat Dept, Via Palermo 26-A, I-43122 Parma, Italy

[4] Swiss Fed Inst Technol, Inst Mol Syst Biol, Otto Stern Weg 3, CH-8093 Zurich, Switzerland

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2020年 / 63卷

关键词：

MASS-SPECTROMETRY; LINKED PEPTIDES; ACIDIC RESIDUES; IDENTIFICATION; VISUALIZATION; INTERFACES; SOFTWARE; MITOCHONDRIA; STRATEGY; PRODUCTS;

D O I：

10.1016/j.copbio.2019.12.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Just recently, chemical cross-linking combined with mass spectrometry (XL-MS) has emerged as valuable tool to study protein interaction networks on the system-wide level. The current challenges in XL-MS are to develop robust workflows enabling a comprehensive capture of dynamic biological assemblies in their native environment in a routine manner. In this review, we will highlight both the latest technological developments as well as selected applications of XL-MS for investigating protein networks in cells, organisms, and tissue. In addition, different bioinformatics tools for data analysis will be presented. In light of these exciting new developments, XL-MS can be expected to become one of the most versatile techniques to address important biological questions in a timely manner.

引用

页码：48 / 53

页数：6

共 74 条

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