Development of a real-time PCR assay for the rapid detection of Acinetobacter baumannii from whole blood samples

被引：0
作者：
De Gregorio, Eliana [1 ]
Roscetto, Emanuela [1 ]
Iula, Vita Dora [1 ]
Martinucci, Marianna [1 ]
Zarrilli, Raffaele [2 ]
Di Nocera, Pier Paolo [1 ]
Catania, Maria Rosario [1 ]
机构：
[1] Univ Naples Federico II, Dept Mol Med & Med Biotechnol, I-80131 Naples, Italy
[2] Univ Naples Federico II, Dept Publ Hlth, I-80131 Naples, Italy
来源：
NEW MICROBIOLOGICA | 2015年 / 38卷 / 02期
关键词：
Bacterial bloodstream infection; Molecular diagnostics; TaqMan real-time PCR; Blood culture; Sepsis; BIOFILM-ASSOCIATED PROTEIN; IDENTIFICATION; DIFFERENTIATION; INFECTIONS; HOSPITALS; PATHOGENS; SEQUENCES; BACTERIAL;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Acinetobacter baumannii is a multidrug-resistant pathogen associated with severe infections in hospitalized patients, including pneumonia, urinary and bloodstream infections. Rapid detection of A. baumannii infection is crucial for timely treatment of septicemic patients. The aim of the present study was to develop a specific marker for a quantitative polymerase chain reaction (PCR) assay for the detection of A. baumannii. The target gene chosen is the biofilm-associated protein (bap) gene, encoding a cell surface protein involved in biofilm formation. The assay is specific for A. baumannii, allowing its discrimination from different species of Acinetobacter and other clinically relevant bacterial pathogens. The assay is able to detect one genomic copy of A. baumannii, corresponding to 4 fg of purified DNA, and 20 colony-forming units/ml using DNA extracted from spiked whole blood samples.
引用
收藏
页码：251 / 257
页数：7
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