Simple and sensitive method for determination of glycoalkaloids in potato tubers by high-performance liquid chromatography with chemiluminescence detection

被引：43
作者：
Kodamatani, H
Saito, K
Niina, N
Yamazaki, S
Tanaka, Y
机构：
[1] Kobe Univ, Fac Human Dev, Div Nat Environm & Chem, Kobe, Hyogo 6578501, Japan
[2] Kobe Univ, Grad Sch Cultural Studies & Human Sci, Kobe, Hyogo 6578501, Japan
[3] Okayama Univ Sci, Dept Appl Sci, Okayama 7000005, Japan
[4] Kobe Univ, Fac Human Dev, Div Hlth Promot, Kobe, Hyogo 6578501, Japan
来源：
JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1100卷 / 01期
关键词：
potato glycoalkaloids; alpha-solanine; alpha-chaconine; chemiluminescence; Ru(bpy)(3)(3+); HPLC;
D O I：
10.1016/j.chroma.2005.09.006
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel, simple and sensitive high-performance liquid chromatographic method for the determination of the potato glycoalkaloids, alpha-solanine and alpha-chaconine, based on the chemiluminescent reaction of tris(2,2'-bipyridine)ruthenium(III) has been developed. The calibration graph was linear in the range of 5 ng/ml-10 mu g/ml for both alpha-solanine and (x-chaconine. The detection limits of alpha-solanine and alpha-chaconine were 1.2 and 1.3 ng/ml, respectively. This method was successfully applied to a potato tuber sample without cleanup, pre-concentration, and derivatization steps. The recoveries (mean standard deviation, %) of alpha-solanine and (x-chaconine spiked in tuber pith at 10 mu g/g (n = 6) were 101.0 +/- 4.4% and 103.6 +/- 7.1%, respectively. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：26 / 31
页数：6
相关论文
共 31 条
[11]   LIQUID-CHROMATOGRAPHY OF ANTIHISTAMINES USING POSTCOLUMN TRIS(2,2'-BIPYRIDINE) RUTHENIUM(III) CHEMILUMINESCENCE DETECTION [J].
HOLEMAN, JA ;
DANIELSON, ND .
JOURNAL OF CHROMATOGRAPHY A, 1994, 679 (02) :277-284
[12]   NATURALLY-OCCURRING TOXIC ALKALOIDS IN FOODS [J].
JADHAV, SJ ;
SHARMA, RP ;
SALUNKHE, DK .
CRC CRITICAL REVIEWS IN TOXICOLOGY, 1981, 9 (01) :21-104
[13]   A novel enzyme biosensor for steroidal glycoalkaloids detection based on pH-sensitive field effect transistors [J].
Korpan, YI ;
Volotovsky, VV ;
Martelet, C ;
Jaffrezic-Renault, N ;
Nazarenko, EA ;
El'skaya, AV ;
Soldatkin, AP .
BIOELECTROCHEMISTRY, 2002, 55 (1-2) :9-11
[14]   DETERMINATION OF POTATO GLYCOALKALOIDS USING ISOTACHOPHORESIS AND COMPARISON WITH A HPLC METHOD [J].
KVASNICKA, F ;
PRICE, KR ;
NG, K ;
FENWICK, GR .
JOURNAL OF LIQUID CHROMATOGRAPHY, 1994, 17 (09) :1941-1951
[15]   ANALYSIS OF SOLANUM ALKALOIDS USING INTERNAL STANDARDIZATION AND CAPILLARY GAS-CHROMATOGRAPHY [J].
LAWSON, DR ;
ERB, WA ;
MILLER, AR .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1992, 40 (11) :2186-2191
[16]   EVALUATION OF USE OF TRIS(2,2'-BIPYRIDYL)RUTHENIUM(III) AS A CHEMILUMINESCENT REAGENT FOR QUANTITATION IN FLOWING STREAMS [J].
LEE, WY ;
NIEMAN, TA .
ANALYTICAL CHEMISTRY, 1995, 67 (11) :1789-1796
[17]   Tris (2,2'-bipyridyl)ruthenium(II) electrogenerated chemiluminescence in analytical science [J].
Lee, WY .
MIKROCHIMICA ACTA, 1997, 127 (1-2) :19-39
[18]  
MORRIS SC, 1984, FOOD TECHNOL AUST, V36, P118
[19]   GENERATION OF CHEMILUMINESCENCE UPON REACTION OF ALIPHATIC-AMINES WITH TRIS(2,2'-BIPYRIDINE)RUTHENIUM(III) [J].
NOFFSINGER, JB ;
DANIELSON, ND .
ANALYTICAL CHEMISTRY, 1987, 59 (06) :865-868
[20]   2,2'-BIPYRIDINE COMPLEXES .I. POLARIZED CRYSTAL SPECTRA OF TRIS(2,2'-BIPYRIDINE)COPPER(2) -NICKEL(2) -COBALT(2) -IRON(2) AND -RUTHENIUM(2) [J].
PALMER, RA ;
PIPER, TS .
INORGANIC CHEMISTRY, 1966, 5 (05) :864-&
1234