Comparasion of five gene loci (rnpB, 16S rRNA, 16S-23S rRNA, sodA and dnaJ) to aid the molecular identification of viridans-group streptococci and pneumococci

被引：12

作者：

Maeda, Y. ^{[1
,2
]}

Goldsmith, C. E. ^{[1
]}

Coulter, W. A. ^{[3
]}

Mason, C. ^{[3
]}

Dooley, J. S. G. ^{[2
]}

Lowery, C. J. ^{[2
]}

Millar, B. C. ^{[1
]}

Moore, J. E. ^{[1
,2
]}

机构：

[1] Belfast City Hosp, Dept Bacteriol, No Ireland Publ Hlth Lab, Belfast BT9 7AD, Antrim, North Ireland

[2] Univ Ulster, Sch Biomed Sci, Coleraine BT52 1SA, Londonderry, North Ireland

[3] Queens Univ Belfast, Sch Dent, Royal Grp Hosp, Belfast, Antrim, North Ireland

来源：

BRITISH JOURNAL OF BIOMEDICAL SCIENCE | 2011年 / 68卷 / 04期

关键词：

Molecular typing; Streptococcus pneumoniae; Viridans streptococci; SEQUENCE-ANALYSIS; PHYLOGENETIC-RELATIONSHIPS; SPECIES IDENTIFICATION; POLYMORPHISM ANALYSIS; ORAL STREPTOCOCCI; PCR; RELEVANT; AMPLIFICATION; STRAINS; LEVEL;

D O I：

10.1080/09674845.2011.11730349

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Viridans-group streptococci (VGS) consist of several taxa which historically have been highly diverse. However, at times it may become necessary to have a reliable scheme for the identification of these organisms to the species level. The aim of this study is to compare the ability of five gene loci, namely rnpB, 16S rRNA, 16S-23S rRNA, sodA and dnaJ, to speciate such organisms through a sequence typing-based approach. Reference organisms consisting of six VGS species were compared based on sequence typing, followed by comparison of 31 wild-type respiratory isolates, and showed that employment of sequence typing using the rnpB gene locus was the most specific and reliable. Therefore, the use of rnpB sequencing for the identification of VGS to species level is a reliable and feasible option, based on a single gene target.

引用

页码：190 / 196

页数：7

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