Light-initiated hydroxylation of lauric acid using hybrid P450 BM3 enzymes

被引：45
作者：
Ngoc-Han Tran [1 ]
Ngoc Huynh [1 ]
Thuba Bui [1 ]
Yen Nguyen [1 ]
Phuong Huynh [1 ]
Cooper, Mary E. [1 ]
Cheruzel, Lionel E. [1 ]
机构：
[1] San Jose State Univ, Dept Chem, San Jose, CA 95192 USA
来源：
CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 43期
基金：
美国国家科学基金会;
关键词：
ELECTRON-TRANSFER; HYDROGEN-PEROXIDE; RU(II) COMPLEXES; BOND ACTIVATION; CYTOCHROME-P450; PHOTOOXIDATION; CATALYSIS; OXIDATION; EFFICIENT; DRIVEN;
D O I：
10.1039/c1cc15124j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We have developed hybrid P450 BM3 enzymes consisting of a Ru(II)-diimine photosensitizer covalently attached to non-native single cysteine residues of P450 BM3 heme domain mutants. These enzymes are capable, upon light activation, of selectively hydroxylating lauric acid with 40 times higher total turnover numbers compared to the peroxide shunt.
引用
收藏
页码：11936 / 11938
页数：3
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