A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I

被引：13

作者：

Xu, Hui ^{[1
]}

Gao, Shuli ^{[1
]}

Liu, Quanwen ^{[1
]}

Pan, Dun ^{[2
]}

Wang, Lihua ^{[2
]}

Ren, Shuzhen ^{[3
]}

Ding, Min ^{[3
]}

Chen, Jingwen ^{[3
]}

Liu, Gang ^{[3
]}

机构：

[1] Ludong Univ, Sch Chem & Mat Sci, Yantai 264025, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China

[3] Shanghai Inst Measurement & Testing Technol, Shanghai 201203, Peoples R China

来源：

SENSORS | 2011年 / 11卷 / 11期

关键词：

cysteine; competition assay; mercury-specific DNA; Hg2+; Sybr Green I; PERFORMANCE LIQUID-CHROMATOGRAPHY; FLOW-INJECTION DETERMINATION; CARBON-PASTE ELECTRODE; GOLD NANOPARTICLES; N-ACETYLCYSTEINE; METAL-COMPLEXES; ASCORBIC-ACID; AMINO-ACIDS; GLUTATHIONE; CONSTANTS;

D O I：

10.3390/s111110187

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We here report a rapid, sensitive, selective and label-free fluorescence detection method for cysteine (Cys). The conformation of mercury-specific DNA (MSD) changes from a random coil form to a hairpin structure in the presence of Hg2+ due to the formation of a thymine-Hg2+-thymine (T-Hg2+-T) complex. Cys can selectively coordinate with Hg2+ and extract it from the thymine-Hg2+-thymine complex. The hairpin structure dehybridizes and the fluorescence intensity of Sybr Green I (SG) decreases upon addition of Cys because SG efficiently discriminates mercury-specific DNA and mercury-specific DNA/Hg2+ complex. The detection can be finished within 5 min with high sensitivity and selectivity. In addition, we can obtain variable dynamic ranges for Cys by changing the concentration of MSD/Hg2+.

引用

页码：10187 / 10196

页数：10

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