A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I

被引：13
作者：
Xu, Hui [1 ]
Gao, Shuli [1 ]
Liu, Quanwen [1 ]
Pan, Dun [2 ]
Wang, Lihua [2 ]
Ren, Shuzhen [3 ]
Ding, Min [3 ]
Chen, Jingwen [3 ]
Liu, Gang [3 ]
机构：
[1] Ludong Univ, Sch Chem & Mat Sci, Yantai 264025, Peoples R China
[2] Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China
[3] Shanghai Inst Measurement & Testing Technol, Shanghai 201203, Peoples R China
来源：
SENSORS | 2011年 / 11卷 / 11期
关键词：
cysteine; competition assay; mercury-specific DNA; Hg2+; Sybr Green I; PERFORMANCE LIQUID-CHROMATOGRAPHY; FLOW-INJECTION DETERMINATION; CARBON-PASTE ELECTRODE; GOLD NANOPARTICLES; N-ACETYLCYSTEINE; METAL-COMPLEXES; ASCORBIC-ACID; AMINO-ACIDS; GLUTATHIONE; CONSTANTS;
D O I：
10.3390/s111110187
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We here report a rapid, sensitive, selective and label-free fluorescence detection method for cysteine (Cys). The conformation of mercury-specific DNA (MSD) changes from a random coil form to a hairpin structure in the presence of Hg2+ due to the formation of a thymine-Hg2+-thymine (T-Hg2+-T) complex. Cys can selectively coordinate with Hg2+ and extract it from the thymine-Hg2+-thymine complex. The hairpin structure dehybridizes and the fluorescence intensity of Sybr Green I (SG) decreases upon addition of Cys because SG efficiently discriminates mercury-specific DNA and mercury-specific DNA/Hg2+ complex. The detection can be finished within 5 min with high sensitivity and selectivity. In addition, we can obtain variable dynamic ranges for Cys by changing the concentration of MSD/Hg2+.
引用
收藏
页码：10187 / 10196
页数：10
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