Chilling ovarian fragments during transportation improves viability and growth of goat preantral follicles cultured in vitro

被引：124

作者：

Chaves, R. N. ^{[1
]}

Martins, F. S. ^{[1
]}

Saraiva, M. V. A. ^{[1
]}

Celestino, J. J. H. ^{[1
]}

Lopes, C. A. P. ^{[1
]}

Correia, J. C. ^{[1
]}

Verde, I. B. Lima ^{[1
]}

Matos, M. H. T. ^{[1
]}

Bao, S. N. ^{[2
]}

Name, K. P. O. ^{[2
]}

Campello, C. C. ^{[1
]}

Silva, J. R. V. ^{[3
]}

Figueiredo, J. R. ^{[1
]}

机构：

[1] Univ Estadual Ceara, Fac Vet Med, LAMOFOPA, PPGCV, BR-60740903 Fortaleza, CE, Brazil

[2] Univ Brasilia, Dept Cell Biol, Electron Microscopy Lab, BR-70910900 Brasilia, DF, Brazil

[3] State Univ Vale Acarau, NUBIS, BR-62040370 Sobral, CE, Brazil

来源：

REPRODUCTION FERTILITY AND DEVELOPMENT | 2008年 / 20卷 / 05期

关键词：

caprine; conservation;

D O I：

10.1071/RD07195

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of the present study was to evaluate the effects of storage of goat ovarian fragments at different temperatures and for different incubation times on the viability and growth of cultured preantral follicles in vitro. Caprine ovaries were collected and divided into 19 fragments, with one fragment being fixed immediately (fresh control). The remaining fragments were placed in minimal essential medium (MEM) and maintained at 4, 20 or 35 degrees C for 2 or 4 h. After each incubation period, some of the fragments were fixed (non-cultured), whereas others were cultured in vitro for 1 or 7 days. Fragments were processed to enable routine histological and transmission electron microscopic examination. After 7 days of culture, only ovarian fragments stored at 4 degrees C for 4 h maintained a percentage of morphologically normal follicles similar to that in the fresh control. For all other treatments groups, there was a significant increase in follicular activation observed. In addition, there was an increase in oocyte and follicular diameter after culture of ovarian cortex that had been chilled previously at 4 degrees C for 2 or 4 h. In conclusion, the present study demonstrated that chilling ovarian fragments at 4 degrees C during transportation is best for maintaining follicle viability and to increase follicular growth during in vitro culture.

引用

页码：640 / 647

页数：8