Effects of trans-10, cis-12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs

被引:11
作者
Jose, A. A. F. B. V. [1 ]
Gama, M. A. S. [2 ]
Lanna, D. D. P. [1 ]
机构
[1] Univ Sao Paulo, Dept Zootecnia, Piracicaba, SP, Brazil
[2] Embrapa Gado de Leite, Empresa Brasileira Pesquisa Agropecuaria, Juiz De Fora, MG, Brazil
来源
GENETICS AND MOLECULAR RESEARCH | 2008年 / 7卷 / 02期
关键词
conjugated linoleic acid; adipose tissue explants; lipogenesis; bioactive fatty acid; swine;
D O I
10.4238/vol7-2gmr417
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of C-14-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 +/- 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 mu M polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 mu M PVA; 3) CLA200: 200 mu M trans-10, cis-12 CLA; 4) CLA50: 50 mu M trans-10, cis-12 CLA, and 5) LA: 200 mu M linoleic acid. Fatty acids were added along with PVA (2: 1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12 CLA decreased FAS activity (P < 0.05), but NADPH-generating enzymes were unaffected by treatments. Consistent with reduction in FAS activity, both lipid synthesis and FAS mRNA abundance were reduced with chronic CLA treatment, pGH increased baseline and stimulated lipolysis (P < 0.05) after 48 h of culture, while CLA treatment had no effect on non-esterified fatty acid release. Results of this study showed that trans-10, cis-12 CLA alters lipogenesis but has no effect on lipolysis in cultures of pig adipose tissue.
引用
收藏
页码:284 / 294
页数:11
相关论文
共 50 条
  • [31] Effect of trans-10, cis-12 isomer of conjugated linoleic acid on boar semen quality after cryopreservation
    Pontes Teixeira, Sofia Margarida
    Nobre Chaveiro, Antonio Eduardo
    Moreira da Silva, Joaquim Fernando
    ANIMAL REPRODUCTION, 2017, 14 (02) : 400 - 405
  • [32] Dose response of milk fat to intravenous administration of the trans-10, cis-12 isomer of conjugated linoleic acid
    Viswanadha, S
    Giesy, JG
    Hanson, TW
    McGuire, MA
    JOURNAL OF DAIRY SCIENCE, 2003, 86 (10) : 3229 - 3236
  • [33] De novo synthesis of trans-10, cis-12 conjugated linoleic acid in oleaginous yeast Yarrowia Lipolytica
    Zhang, Baixi
    Rong, Chunchi
    Chen, Haiqin
    Song, Yuanda
    Zhang, Hao
    Chen, Wei
    MICROBIAL CELL FACTORIES, 2012, 11
  • [34] De novo synthesis of trans-10, cis-12 conjugated linoleic acid in oleaginous yeast Yarrowia Lipolytica
    Baixi Zhang
    Chunchi Rong
    Haiqin Chen
    Yuanda Song
    Hao Zhang
    Wei Chen
    Microbial Cell Factories, 11
  • [35] Trans-10,cis-12 conjugated linoleic acid suppresses the desaturation of linoleic and α-linolenic acids in HepG2 cells
    Eder, K
    Slomma, N
    Becker, K
    JOURNAL OF NUTRITION, 2002, 132 (06) : 1115 - 1121
  • [36] Effects of trans-10,cis-12 conjugated linoleic acid and cognates on apolipoprotein B secretion in HepG2 cells
    Storkson, JM
    Park, Y
    Cook, ME
    Pariza, MW
    NUTRITION RESEARCH, 2005, 25 (04) : 387 - 399
  • [37] Hepatomegaly Induced by Trans-10,cis-12 Conjugated Linoleic Acid in Adult Hamsters Fed an Atherogenic Diet Is Not Associated with Steatosis
    Miranda, Jonatan
    Fernandez-Quintela, Alfredo
    Churruca, Itziar
    Rodriguez, Victor M.
    Simon, Edurne
    Portillo, Maria P.
    JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION, 2009, 28 (01) : 43 - 49
  • [38] The effects of conjugated linoleic acid isomers cis-9,trans-11 and trans-10,cis-12 on in vitro bovine embryo production and cryopreservation
    Absalon-Medina, V. A.
    Bedford-Guaus, S. J.
    Gilbert, R. O.
    Siqueira, L. C.
    Esposito, G.
    Schneider, A.
    Cheong, S. H.
    Butler, W. R.
    JOURNAL OF DAIRY SCIENCE, 2014, 97 (10) : 6164 - 6176
  • [39] A conjugated linoleic acid supplement containing trans-10, cis-12 reduces milk fat synthesis in lactating sheep
    Lock, AL
    Teles, BM
    Perfield, JW
    Bauman, DE
    Sinclair, LA
    JOURNAL OF DAIRY SCIENCE, 2006, 89 (05) : 1525 - 1532
  • [40] Fat area and lipid droplet morphology of porcine oocytes during in vitro maturation with trans-10, cis-12 conjugated linoleic acid and forskolin
    Prates, E. G.
    Marques, C. C.
    Baptista, M. C.
    Vasques, M. I.
    Carolino, N.
    Horta, A. E. M.
    Charneca, R.
    Nunes, J. T.
    Pereira, R. M.
    ANIMAL, 2013, 7 (04) : 602 - 609