SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes

被引：1755

作者：

Sungnak, Waradon ^{[1
]}

Huang, Ni ^{[1
]}

Becavin, Christophe ^{[2
]}

Berg, Marijn ^{[3
,4
,5
]}

Queen, Rachel ^{[6
]}

Litvinukova, Monika ^{[1
,7
]}

Talavera-Lopez, Carlos ^{[1
]}

Maatz, Henrike ^{[7
]}

Reichart, Daniel ^{[8
]}

Sampaziotis, Fotios ^{[9
,10
,11
]}

Worlock, Kaylee B. ^{[12
]}

Yoshida, Masahiro ^{[12
]}

Barnes, Josephine L. ^{[12
]}

机构：

[1] Wellcome Sanger Inst, Cambridge, England

[2] Univ Cote Azur, CNRS, IPMC, Sophia Antipolis, France

[3] Univ Groningen, Univ Med Ctr Groningen, Dept Pathol & Med Biol, Groningen, Netherlands

[4] Univ Groningen, Univ Med Ctr Groningen, Groningen Res Inst Asthma, Groningen, Netherlands

[5] Univ Groningen, Univ Med Ctr Groningen, COPD, Groningen, Netherlands

[6] Newcastle Univ, Fac Med Sci, Biosci Inst, Bioinformat Core Facil, Newcastle Upon Tyne, Tyne & Wear, England

[7] Helmholtz Assoc MDC, Max Delbruck Ctr Mol Med, Cardiovasc & Metab Sci, Berlin, Germany

[8] Harvard Med Sch, Dept Genet, Boston, MA 02115 USA

[9] Univ Cambridge, Wellcome & MRC Cambridge Stem Cell Inst, Cambridge, England

[10] Addenbrookes Hosp, Dept Med, Cambridge, England

[11] Cambridge Univ Hosp, Cambridge Liver Unit, Cambridge, England

[12] UCL, Div Med, UCL Resp, London, England

来源：

NATURE MEDICINE | 2020年 / 26卷 / 05期

基金：

新加坡国家研究基金会; 英国惠康基金; 美国国家卫生研究院; 欧盟地平线“2020”; 英国生物技术与生命科学研究理事会; 中国国家自然科学基金; 英国工程与自然科学研究理事会;

关键词：

FUNCTIONAL RECEPTOR; CORONAVIRUS; PROTEIN; ACE2;

D O I：

10.1038/s41591-020-0868-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated SARS-CoV-2 potential tropism by surveying expression of viral entry-associated genes in single-cell RNA-sequencing data from multiple tissues from healthy human donors. We co-detected these transcripts in specific respiratory, corneal and intestinal epithelial cells, potentially explaining the high efficiency of SARS-CoV-2 transmission. These genes are co-expressed in nasal epithelial cells with genes involved in innate immunity, highlighting the cells' potential role in initial viral infection, spread and clearance. The study offers a useful resource for further lines of inquiry with valuable clinical samples from COVID-19 patients and we provide our data in a comprehensive, open and user-friendly fashion at www.covid19cellatlas.org.

引用

页码：681 / +

页数：12

共 50 条

[21] SARS-CoV-2 infection and the antiviral innate immune response
Yang, Hui
Lyu, Yingying
Hou, Fajian
JOURNAL OF MOLECULAR CELL BIOLOGY, 2020, 12 (12) : 963 - 967
[22] Evolution of enhanced innate immune evasion by SARS-CoV-2
Thorne, Lucy G.
Bouhaddou, Mehdi
Reuschl, Ann-Kathrin
Zuliani-Alvarez, Lorena
Polacco, Ben
Pelin, Adrian
Batra, Jyoti
Whelan, Matthew V. X.
Hosmillo, Myra
Fossati, Andrea
Ragazzini, Roberta
Jungreis, Irwin
Ummadi, Manisha
Rojc, Ajda
Turner, Jane
Bischof, Marie L.
Obernier, Kirsten
Braberg, Hannes
Soucheray, Margaret
Richards, Alicia
Chen, Kuei-Ho
Harjai, Bhavya
Memon, Danish
Hiatt, Joseph
Rosales, Romel
McGovern, Briana L.
Jahun, Aminu
Fabius, Jacqueline M.
White, Kris
Goodfellow, Ian G.
Takeuchi, Yasu
Bonfanti, Paola
Shokat, Kevan
Jura, Natalia
Verba, Klim
Noursadeghi, Mahdad
Beltrao, Pedro
Kellis, Manolis
Swaney, Danielle L.
Garcia-Sastre, Adolfo
Jolly, Clare
Towers, Greg J.
Krogan, Nevan J.
NATURE, 2022, 602 (7897) : 487 - +
[23] Evolution of enhanced innate immune evasion by SARS-CoV-2
Lucy G. Thorne
Mehdi Bouhaddou
Ann-Kathrin Reuschl
Lorena Zuliani-Alvarez
Ben Polacco
Adrian Pelin
Jyoti Batra
Matthew V. X. Whelan
Myra Hosmillo
Andrea Fossati
Roberta Ragazzini
Irwin Jungreis
Manisha Ummadi
Ajda Rojc
Jane Turner
Marie L. Bischof
Kirsten Obernier
Hannes Braberg
Margaret Soucheray
Alicia Richards
Kuei-Ho Chen
Bhavya Harjai
Danish Memon
Joseph Hiatt
Romel Rosales
Briana L. McGovern
Aminu Jahun
Jacqueline M. Fabius
Kris White
Ian G. Goodfellow
Yasu Takeuchi
Paola Bonfanti
Kevan Shokat
Natalia Jura
Klim Verba
Mahdad Noursadeghi
Pedro Beltrao
Manolis Kellis
Danielle L. Swaney
Adolfo García-Sastre
Clare Jolly
Greg J. Towers
Nevan J. Krogan
Nature, 2022, 602 : 487 - 495
[24] microRNA, the Innate-Immune System and SARS-CoV-2
Hill, James M.
Lukiw, Walter J.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2022, 12
[25] Evolution of enhanced innate immune evasion in lung epithelial cells by the SARS-CoV-2 B.1.1.7 variant
Thorne, Lucy
Bouhaddou, Mehdi
Reuschl, Ann Kathrin
Alvarez, Lorena Zuliani
Ben Polacco
Pelini, Adrian
Batra, Jyoti
Whelan, Matthew V.
Ummadi, Manisha
Rojc, Ajda
Turner, Jane
Obernier, Kirsten
Braberg, Hannes
Goodfellow, Ian
Jura, Natalia
Verba, Klim
Noursadeghi, Mahdad
Beltrano, Pedro
Swaney, Danielle
Sastre, Adolfo Garcia
Jolly, Clare
Towers, Greg J.
Krogan, Nevan J.
EUROPEAN JOURNAL OF IMMUNOLOGY, 2021, 51 : 113 - 113
[26] Inhibition of acid sphingomyelinase by ambroxol prevents SARS-CoV-2 entry into epithelial cells
Carpinteiro, Alexander
Gripp, Barbara
Hoffmann, Markus
Poehlmann, Stefan
Hoertel, Nicolas
Edwards, Michael J.
Kamler, Markus
Kornhuber, Johannes
Becker, Katrin Anne
Gulbins, Erich
JOURNAL OF BIOLOGICAL CHEMISTRY, 2021, 296
[27] Innate Immune Response to SARS-CoV-2 Infection: From Cells to Soluble Mediators
Ricci, Daniela
Etna, Marilena Paola
Rizzo, Fabiana
Sandini, Silvia
Severa, Martina
Coccia, Eliana Marina
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (13)
[28] Innate and adaptive immunity to SARS-CoV-2 and predisposing factors
Shen, Jiaying
Fan, Junyan
Zhao, Yue
Jiang, Doming
Niu, Zheyun
Zhang, Zihan
Cao, Guangwen
FRONTIERS IN IMMUNOLOGY, 2023, 14
[29] Effect of SARS-CoV-2 Entry Factors on Myeloid Cancers
Alshareef, Abdulraheem
JOURNAL OF NIPPON MEDICAL SCHOOL, 2022, 89 (01) : 95 - 101
[30] High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells
Ma, Ruiqi
Gan, Lu
Jiang, Sanjie
Ding, Peiwen
Chen, Dongsheng
Wu, Jihong
Qian, Jiang
EXPERIMENTAL EYE RESEARCH, 2021, 205

← 1 2 3 4 5 →