Adrenomedullin protects rat cerebral endothelial cells from oxidant damage in vitro

被引：17
作者：
Chen, L
Kis, B
Busija, DW
Yamashita, H
Ueta, Y
机构：
[1] Univ Occupat & Environm Hlth, Sch Med, Dept Physiol, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan
[2] Wake Forest Univ Hlth Sci, Dept Physiol & Pharmacol, Winston Salem, NC 27157 USA
[3] Kyushu Nutr Welf Univ, Kitakyushu, Fukuoka 8038511, Japan
来源：
REGULATORY PEPTIDES | 2005年 / 130卷 / 1-2期
关键词：
adrenomedullin; rat cerebral endothelial cell; hydrogen peroxide; permeability; viability;
D O I：
10.1016/j.regpep.2005.03.006
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Increased permeability and reduced cerebral endothelial cell (CEC) viability induced by oxidative stress are the hallmarks of the blood-brain barrier disruption. In Our experiments hydrogen peroxide (H2O2, 0.5 mM) induced a continuous decrease of the transendothelial electrical resistance (TEER) and resulted in intercellular gap formations in cultured rat CECs. Adrenomedullin (AM) increased TEER, enhanced peripheral localization of F-actin hands and attenuated the increased permeability induced by H2O2. Furthermore, AM treatment preserved mitochondrial membrane potential, attenuated cytochrome c release, and consequently improved CEC viability in H2O2 treated cultures. These results suggest that AM treatment protects CECs against oxidative injury. (c) 2005 Elsevier B.V. All rights reserved.
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收藏
页码：27 / 34
页数：8
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