Electrokinetic acceleration of DNA hybridization in microsystems

被引:5
作者
Lei, Kin Fong [1 ,2 ]
Wang, Yun-Hsiang [1 ]
Chen, Huai-Yi [3 ,4 ,5 ,6 ]
Sun, Jia-Hong [2 ]
Cheng, Ji-Yen [6 ]
机构
[1] Chang Gung Univ, Grad Inst Med Mechatron, Taoyuan 333, Taiwan
[2] Chang Gung Univ, Dept Mech Engn, Taoyuan 333, Taiwan
[3] Natl Tsing Hua Univ, Dept Engn & Syst Sci, Taipei, Taiwan
[4] Acad Sinica, Taiwan Int Grad Program, Nano Sci & Technol Program, Taipei, Taiwan
[5] Natl Tsing Hua Univ, Taipei, Taiwan
[6] Acad Sinica, Res Ctr Appl Sci, Taipei, Taiwan
关键词
DNA hybridization; Electrokinetics; Microfluidics; Genetic diagnostics; FLUID-FLOW; ENHANCEMENT; DEVICES; FIELDS; ARRAY;
D O I
10.1016/j.talanta.2015.02.024
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this work, electrokinetic acceleration of DNA hybridization was investigated by different combinations of frequencies and amplitudes of actuating electric signals. Because the frequencies from low to high can induce different kinds of electrokinetic forces, i.e., electroosmotic to electrothermal forces, this work provides an in-depth investigation of electrokinetic enhanced hybridization. Concentric circular Cr/Au microelectrodes of 350 mu m in diameter were fabricated on a glass substrate and probe DNA was immobilized on the electrode surface. Target DNA labeled with fluorescent dyes suspending in solution was then applied to the electrode. Different electrokinetic forces were induced by the application of different electric signals to the circular microelectrodes. Local microfluidic vortexes were generated to increase the collision efficiency between the target DNA suspending in solution and probe DNA immobilized on the electrode surface. DNA hybridization on the electrode surface could be accelerated by the electrokinetic forces. The level of hybridization was represented by the fluorescent signal intensity ratio. Results revealed that such 5-min dynamic hybridization increased 4.5 fold of signal intensity ratio as compared to a 1-h static hybridization. Moreover, dynamic hybridization was found to have better differentiation ability between specific and non-specific target DNA. This study provides a strategy to accelerate DNA hybridization in microsystems. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:149 / 154
页数:6
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