Virus Isolation from the First Patient with SARS-CoV-2 in Korea

被引：121

作者：

Park, Wan Beom ^{[1
,2
]}

Kwon, Nak-Jung ^{[3
]}

Choi, Su-Jin ^{[2
]}

Kang, Chang Kyung ^{[1
]}

Choe, Pyoeng Gyun ^{[1
]}

Kim, Jin Yong ^{[4
]}

Yun, Jiyoung ^{[3
]}

Lee, Gir-Won ^{[3
]}

Seong, Moon-Woo ^{[5
]}

Kim, Nam Joong ^{[1
,2
]}

Seo, Jeong-Sun ^{[3
,6
]}

Oh, Myoung-don ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, Dept Internal Med, Coll Med, 101 Daehak Ro, Seoul 03080, South Korea

[2] Seoul Natl Univ Hosp, Lab Infect & Immun, Biomed Res Inst, Seoul, South Korea

[3] Macrogen Inc, Seoul, South Korea

[4] Incheon Med Ctr, Dept Internal Med, Incheon, South Korea

[5] Seoul Natl Univ, Dept Lab Med, Coll Med, Seoul, South Korea

[6] Seoul Natl Univ, Precis Med Ctr, Bundang Hosp, Seongnam, South Korea

来源：

JOURNAL OF KOREAN MEDICAL SCIENCE | 2020年 / 35卷 / 07期

关键词：

2019 Novel Coronavirus; Culture; Microscopy; Electron; Phylogeny; Korea; CORONAVIRUS; ALIGNMENT;

D O I：

10.3346/jkms.2020.35.e84

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARSCoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.

引用

页数：5

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