FK409 decomposes and releases nitric oxide (NO) spontaneously when it is dissolved in phosphate buffer solution at 37 degreesC, With the use of this NO donor, the effect of exogenous NO on cardiac contractility was examined by assessing Emax, alpha -chloralose-anaesthetized dogs were instrumented for measurements of left ventricular (LV) pressure and volume and coronary blood flow (CBF) in the left anterior descending artery (LAD), FK409, 8-bromoguanosine-cyclic-monophosphate (8-Br-cGMP) and papaverine were infused into the LAD, and Emax was determined by transient inferior vena cava occlusion when CBF was increased and reached its peak. Neither drug affected heart rate nor LV pressure just before the measurement of Emax, FI(409 increased CBF and decreased Emax in a dose-dependent manner. 8-Br-cGMP also increased CBF and decreased Emax in a dose-dependent manner, Pretreating with propranolol did not affect the effects of FK4098-Br-cGMP on CBF and Emax, Papaverine increased mean CBF but did not affect Emax, In conclusion NO attenuates cardiac contractility in vivo, while increasing CBF, This effect seems to be mediated by cyclic-guanosine monophosphate, a second messenger of NO.
