Magnetic levitating polymeric nano/microparticular substrates for three-dimensional tumor cell culture

被引：17

作者：

Lee, Woong Ryeol ^{[1
]}

Oh, Kyung Taek ^{[2
]}

Park, So Young ^{[1
]}

Yoo, Na Young ^{[1
]}

Ahn, Yong Sik ^{[1
]}

Lee, Don Haeng ^{[3
]}

Youn, Yu Seok ^{[4
]}

Lee, Deok-Keun ^{[5
]}

Cha, Kyung-Hoi ^{[5
]}

Lee, Eun Seong ^{[1
]}

机构：

[1] Catholic Univ Korea, Div Biotechnol, Bucheon Si 420743, Gyeonggi Do, South Korea

[2] Chung Ang Univ, Coll Pharm, Seoul 155756, South Korea

[3] Inha Univ, Dept Internal Med, Inchon 402751, South Korea

[4] Pusan Natl Univ, Coll Pharm, Pusan 609735, South Korea

[5] Dongkook Pharm Co, Gyeonggi Bioctr, Suwon 443270, South Korea

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2011年 / 85卷 / 02期

关键词：

PLGA microparticle; PLLA-b-PEG-folate nanoparticle; Iron oxide; Tumor spheroid; IN-VITRO; PH; NANOPARTICLES; SCAFFOLD; GROWTH;

D O I：

10.1016/j.colsurfb.2011.02.021

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Herein, we describe magnetic cell levitation models using conventional polymeric microparticles or nanoparticles as a substrate for the three-dimensional tumor cell culture. When the magnetic force originating from the ring-shaped magnets overcame the gravitational force, the magnetic field-levitated KB tumor cells adhered to the surface area of magnetic iron oxide (Fe3O4)-encapsulated nano/microparticles and concentrated clusters of levitated cells, ultimately developing tumor cells to tumor spheroids. These simple cell culture models may prove useful for the screening of anticancer drugs and their formulations. (C) 2011 Elsevier By. All rights reserved.

引用

页码：379 / 384

页数：6

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