Attenuation of Brain Nitrosative and Oxidative Damage by Brain Cooling during Experimental Traumatic Brain Injury

被引:19
作者
Kuo, Jinn-Rung [2 ,3 ,4 ]
Lo, Chong-Jeh [2 ,5 ,6 ]
Chang, Ching-Ping [3 ]
Lin, Mao-Tsun [1 ,7 ]
Chio, Chung-Ching [4 ]
机构
[1] Chi Mei Med Ctr, Dept Med Res, Tainan 710, Taiwan
[2] Natl Cheng Kung Univ, Inst Clin Med, Sch Med, Tainan 704, Taiwan
[3] So Taiwan Univ, Dept Biotechnol, Tainan 710, Taiwan
[4] Chi Mei Med Ctr, Dept Surg, Tainan 710, Taiwan
[5] Chang Gung Mem Hosp, Div Trauma & Emergency Surg, Kaohsiung 833, Taiwan
[6] Kaohsiung Med Ctr, Kaohsiung 833, Taiwan
[7] Taipei Med Univ, Grad Inst Dis Prevent & Control, Taipei 112, Taiwan
来源
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2011年
关键词
THERAPEUTIC HYPOTHERMIA; STRESS; ISCHEMIA; INFUSION; STROKE; RATS; NEUROPROTECTION; DYSFUNCTION; APOPTOSIS; LESSONS;
D O I
10.1155/2011/145214
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The aim of the present study was to ascertain whether brain cooling causes attenuation of traumatic brain injury by reducing brain nitrostative and oxidative damage. Brain cooling was accomplished by infusion of 5mL of 4 degrees C saline over 5 minutes via the external jugular vein. Immediately after the onset of traumatic brain injury, rats were randomized into two groups and given 37 degrees C or 4 degrees C normal saline. Another group of rats were used as sham operated controls. Behavioral and biochemical assessments were conducted on 72 hours after brain injury or sham operation. As compared to those of the sham-operated controls, the 37 degrees C saline-treated brain injured animals displayed motor deficits, higher cerebral contusion volume and incidence, higher oxidative damage (e. g., lower values of cerebral superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, but higher values of cerebral malondialdehyde), and higher nitrostative damage (e. g., higher values of neuronal nitric oxide synthase and 3-nitrotyrosine). All the motor deficits and brain nitrostative and oxidative damage were significantly reduced by retrograde perfusion of 4 degrees C saline via the jugular vein. Our data suggest that brain cooling may improve the outcomes of traumatic brain injury in rats by reducing brain nitrostative and oxidative damage.
引用
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页数:8
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