Pyrobayes:: an improved base caller for SNP discovery in pyrosequences

被引：137

作者：

Quinlan, Aaron R. ^{[1
]}

Stewart, Donald A. ^{[1
]}

Stromberg, Michael P. ^{[1
]}

Marth, Gabor T. ^{[1
]}

机构：

[1] Boston Coll, Dept Biol, Chestnut Hill, MA 02467 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 02期

关键词：

D O I：

10.1038/NMETH.1172

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Previously reported applications of the 454 Life Sciences pyrosequencing technology have relied on deep sequence coverage for accurate polymorphism discovery because of frequent insertion and deletion sequence errors. Here we report a new base calling program, Pyrobayes, for pyrosequencing reads. Pyrobayes permits accurate single-nucleotide polymorphism (SNP) calling in resequencing applications, even in shallow read coverage, primarily because it produces more confident base calls than the native base calling program.

引用

页码：179 / 181

页数：3

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