Identification of plant tissues from the gut of Phlebotomus papatasi by DNA analysis

被引：13

作者：

Junnila, Amy ^{[1
]}

Mueller, Gunter C. ^{[1
]}

Schlein, Yosef ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Dept Microbiol & Mol Genet, Inst Med Res Israel Canada, Kuvin Ctr Study Infect & Trop Dis,Fac Med, IL-91120 Jerusalem, Israel

来源：

ACTA TROPICA | 2011年 / 117卷 / 01期

关键词：

Phlebotomus papatasi; rbcL; Suaeda asphaltica; Leishmaniasis; Israel; LEISHMANIA-MAJOR; JORDAN VALLEY; SUGAR MEALS; DIPTERA; PSYCHODIDAE; FOCUS; MOSQUITOS; NECTAR; ISRAEL; DIET;

D O I：

10.1016/j.actatropica.2010.08.019

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Sugar and cellulose assays can determine if an insect has fed on sugar and plant tissue but they cannot identify the species of source plant. In this study, we used DNA analysis together with traditional cellulose and sugar assays to examine the plant feeding habits of wild Phlebotomus papatasi during the dry season in the Lower Jordan Valley, Israel. About 37% of the sand flies were positive for sugar, 45% were positive for cellulose in the gut, and 41% contained amplifiable plant DNA. All of the plant DNA fragments sequenced from both males and females were identified as Suaeda asphaltica. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：14 / 18

页数：5

共 29 条

[1] The recent emergence of Leishmania tropica in Jericho (A'riha) and its environs, a classical focus of L-major [J].