Exploring the anti-proliferative, pro-apoptotic, and antioxidant properties of Santolina corsica Jord. & Fourr. (Asteraceae)

被引：24

作者：

Bonesi, Marco ^{[1
]}

Brindisi, Matteo ^{[1
]}

Armentano, Biagio ^{[1
]}

Curcio, Rosita ^{[1
]}

Sicari, Vincenzo ^{[2
]}

Loizzo, Monica R. ^{[1
]}

Cappello, Maria S. ^{[3
]}

Bedini, Gianni ^{[4
]}

Peruzzi, Lorenzo ^{[4
]}

Tundis, Rosa ^{[1
]}

机构：

[1] Univ Calabria, Dept Pharm Hlth & Nutr Sci, Via P Bucci, I-87036 Arcavacata Di Rende, CS, Italy

[2] Univ Mediterranea Reggio Calabria, Dept Agr, I-89122 Reggio Di Calabria, Italy

[3] CNR, Inst Sci Food Prod ISPA, I-73100 Lecce, Italy

[4] Univ Pisa, Dept Biol, Unit Bot, Via Derna 1, I-56126 Pisa, Italy

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2018年 / 107卷

关键词：

Santolina corsica Jord. & Fourr; Antioxidant activity; Anti-proliferative activity; Anti-inflammatory activity; Invasivity; Apoptosis; BREAST-CANCER CELLS; OXIDATIVE DAMAGE; ESSENTIAL OILS; AERIAL PARTS; SESQUITERPENES; INFLAMMATION; GENE;

D O I：

10.1016/j.biopha.2018.08.090

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Aims: The bioactivities of Santolina corsica Jord. & Fourr. n-hexane (EHS) and methanol (EMS) extracts were evaluated in relation to their chemical profile. Main methods: EHS and EMS were analysed by gas chromatography-mass spectrometry () and high performance liquid chromatography-diode array detection (HPLC-DAD), respectively. Antioxidant activity was determined by beta-carotene bleaching, Ferric Reducing Activity Power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) tests. Nitric oxide (NO) production was assessed in LPS-stimulated RAW 264.7 cells. Anti-proliferative activity was evaluated by MTT assay on A549, HeLa, PC3, MCF-7, MDA-MB-231 cancer cells, and non-tumorigenic MCF10 A cells. Cell motility, migration and invasion were assessed by wound-healing scratch, migration and invasion assays, respectively. DNA fragmentation was tested by TUNEL assay. Cells morphology was studied by phase-contrast microscopy. Procaspase-8, -9, poly (ADP-ribose) polymerase and COX-2 expression levels were evaluated by immunoblotting analysis. Key findings: Kaempferol-3-O-glucoside (5878.67 mg/100 g of extract), chlorogenic acid (746.11 mg/100 g), and rosmarinic acid (550.16 mg/100 g) were the dominant EMS constituents. EHS showed myrcene (18.86%) as the main compound, followed by palmitic acid methyl and ethyl esters (9.35 and 9.16%, respectively), beta-phellandrene (8.48%), and ar-curcumene (5.63%). Both extracts showed promising anti-proliferative activity on all tested cancer cells, without inducing cytotoxicity in non-tumorigenic cells MCF-10 A. Moreover, extracts inhibited motility, migration, and invasion of MDA-MB-231 cells, inducing apoptosis. EHS decreased NO production, showing anti-inflammatory activity. Significance: S. corsica extracts might be potentially useful in cancer treatment, since reduce invasive and migratory potential of MDA-MB-231 cells triggering apoptosis.

引用

页码：967 / 978

页数：12

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