Helicobacter pylori Infection Promotes Methylation and Silencing of Trefoil Factor 2, Leading to Gastric Tumor Development in Mice and Humans

被引:120
作者
Peterson, Anthony J.
Menheniott, Trevelyan R.
O'Connor, Louise
Walduck, Anna K. [2 ]
Fox, James G. [3 ]
Kawakami, Kazuyuki [4 ]
Minamoto, Toshinari [4 ]
Ong, Eng Kok [1 ]
Wang, Timothy C. [5 ]
Judd, Louise M. [6 ]
Giraud, Andrew S. [1 ,6 ]
机构
[1] Royal Childrens Hosp, Murdoch Childrens Res Inst, Sequenom Platform Facil, Parkville, Vic 3052, Australia
[2] Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3052, Australia
[3] MIT, Div Comparat Med, Dept Biol Engn, Cambridge, MA 02139 USA
[4] Kanazawa Univ, Canc Res Inst, Div Translat & Clin Oncol, Kanazawa, Ishikawa 920, Japan
[5] Columbia Univ, Sch Med, Div Digest & Liver Dis, New York, NY USA
[6] Univ Melbourne, Royal Childrens Hosp, Dept Paediat, Parkville, Vic 3052, Australia
基金
英国医学研究理事会;
关键词
Tumor Suppressor; Epigenetics; Stomach Cancer; Trefoil Factor 2; HUMAN SPASMOLYTIC POLYPEPTIDE; ACUTE OXYNTIC ATROPHY; GENE-EXPRESSION; INTESTINAL METAPLASIA; CELL-MIGRATION; MUTANT MICE; CANCER; PEPTIDES; PROTEIN; MUCOSA;
D O I
10.1053/j.gastro.2010.08.043
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
BACKGROUND & AIMS: Trefoil factors (TFFs) regulate mucosal repair and suppress tumor formation in the stomach. Tff1 deficiency results in gastric cancer, whereas Tff2 deficiency increases gastric inflammation. TFF2 expression is frequently lost in gastric neoplasms, but the nature of the silencing mechanism and associated impact on tumorigenesis have not been determined. METHODS: We investigated the epigenetic silencing of TFF2 in gastric biopsy specimens from individuals with Helicobacter pylori-positive gastritis, intestinal metaplasia, gastric cancer, and disease-free controls. TFF2 function and methylation were manipulated in gastric cancer cell lines. The effects of Tff2 deficiency on tumor growth were investigated in the gp130(F/F) mouse model of gastric cancer. RESULTS: In human tissue samples, DNA methylation at the TFF2 promoter began at the time of H pylori infection and increased throughout gastric tumor progression. TFF2 methylation levels were inversely correlated with TFF2 messenger RNA levels and could be used to discriminate between disease-free controls, H pylori-infected, and tumor tissues. Genome demethylation restored TFF2 expression in gastric cancer cell lines, so TFF2 silencing requires methylation. In Tff2-deficient gp130(F/F)/Tff2(-/-) mice, proliferation of mucosal cells and release of T helper cell type-1 (Th-1) 1 cytokines increased, whereas expression of gastric tumor suppressor genes and Th-2 cytokines were reduced, compared with gp130(F/F)controls. The fundus of gp130(F/F)/Tff2(-/-) mice displayed glandular atrophy and metaplasia, indicating accelerated preneoplasia. Experimental H pylori infection in wild-type mice reduced antral expression of Tff2 by increased promoter methylation. CONCLUSIONS: TFF2 negatively regulates preneoplastic progression and subsequent tumor development in the stomach, a role that is subverted by promoter methylation during H pylori infection.
引用
收藏
页码:2005 / 2017
页数:13
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