Non-contact fluorescent bleaching-independent method for temperature measurement in microfluidic systems based on DNA melting curves

被引：13

作者：

Neuzil, Pavel ^{[1
]}

Cheng, Fang ^{[1
]}

Soon, Jeffrey Bo Woon ^{[1
]}

Qian, Lan Liu ^{[1
]}

Reboud, Julien ^{[1
]}

机构：

[1] Inst Microelect, Singapore, Singapore

来源：

LAB ON A CHIP | 2010年 / 10卷 / 20期

关键词：

POLYMERASE-CHAIN-REACTION; DYE;

D O I：

10.1039/c005243d

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This report introduces a bleaching-independent temperature measurement method based on the analysis of the fluorescence emitted during the melting of DNA molecules with the SYBR-Green I intercalator, in a microvolume where the strong non-linearity of the signal is used to eliminate the photobleaching effect as well as to determine the heat transfer rate between a heater and the sample and the temperature non-uniformity within the sample.

引用

收藏

页码：2818 / 2821

页数：4

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