Comparison of the oxidative stress response of two Antarctic fungi to different growth temperatures

被引：6

作者：

Kostadinova, Nedelina ^{[1
]}

Tosi, Solveig ^{[2
]}

Spassova, Boryana ^{[1
]}

Angelova, Maria ^{[1
]}

机构：

[1] Bulgarian Acad Sci, Stephan Angeloff Inst Microbiol, Academician G Bonchev 26, BU-1113 Sofia, Bulgaria

[2] Univ Pavia, Dept Earth & Environm Sci, Via S Epifanio 14, I-27100 Pavia, Italy

来源：

POLISH POLAR RESEARCH | 2017年 / 38卷 / 03期

关键词：

Antarctic; Livingston Island; fungi; physiological cell response; trehalose; glycogen; antioxidant enzymes; SUPEROXIDE-DISMUTASE; SACCHAROMYCES-CEREVISIAE; ANTIOXIDANT RESPONSE; COLD STRESS; DIVERSITY; COMMUNITIES; GLUCOSE; ENZYMES; ADAPTATION; METABOLISM;

D O I：

10.1515/popore-2017-0015

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Two fungal strains, isolated from Livingston Island, Antarctica (Penicillium commune 161, psychrotolerant and Aspergillus glaucus 363, mesophilic) were investigated for a relationship between growth temperature and oxidative stress response. Cultivation at temperatures below - (10 and 15 degrees C and 10 and 20 degrees C for P. commune and A. glaucus, respectively) and above (25 degrees C and 30 degrees C for P. commune and A. glaucus, respectively) the optimum caused significant difference in growth and glucose uptake in comparison with the control cultures. Enhanced level of reserve carbohydrates (glycogen and trehalose) was determined under cultivation at different temperatures from the optimal one. While the highest content of trehalose was found in the exponential phase, glycogen accumulation was observed in the stationary phase when growth conditions deteriorate. The growth at temperature below- and above-optimum caused strain-dependent changes in two antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). While SOD activity in the psychrotolerant strain increases with decreasing of growth temperature, the mesophilic A. glaucus demonstrated marked reduction of it at below- and above-optimal temperature. Decreasing trend of CAT activity was observed in both strains below the optimal temperature indicating a lack of antioxidant protection from this enzyme under the cold stress conditions.

引用

页码：393 / 408

页数：16

共 60 条

[11] Dreesens Lisa L, 2014, Biology (Basel), V3, P466, DOI 10.3390/biology3030466
[12] Ellis-Evans J C., 1985, Antarctic nutrient cycles and food webs, P662
[13] Feller G, 1996, FEMS MICROBIOL REV, V18, P189, DOI 10.1016/0168-6445(96)00011-3
[14] The Psychrotolerant Antarctic Fungus Lecanicillium muscarium CCFEE 5003: A Powerful Producer of Cold-Tolerant Chitinolytic Enzymes
Fenice, Massimiliano
[J]. MOLECULES, 2016, 21 (04):
[15] Combined effects of agitation and aeration on the chitinolytic enzymes production by the Antarctic fungus Lecanicillium muscarium CCFEE 5003
Fenice, Massimiliano
Barghini, Paolo
Selbmann, Laura
Federici, Federico
[J]. MICROBIAL CELL FACTORIES, 2012, 11
[16] Different mechanisms of the biochemical adaptation of mycelial fungi to temperature stress: Changes in the cytosol carbohydrate composition
Feofilova, EP
Tereshina, VM
Khokhlova, NS
Memorskaya, AS
[J]. MICROBIOLOGY, 2000, 69 (05) : 504 - 508
[17] Fridovich I, 1998, J EXP BIOL, V201, P1203
[18] Frisvad Jens C., 2008, P137, DOI 10.1007/978-3-540-74335-4_9
[19] MEMBRANE LIPID-COMPOSITION AND GLUCOSE-UPTAKE IN 2 PSYCHROTOLERANT BACTERIA FROM ANTARCTICA
FUKUNAGA, N
RUSSELL, NJ
[J]. JOURNAL OF GENERAL MICROBIOLOGY, 1990, 136 : 1669 - 1673
[20] Temperature downshift induces antioxidant response in fungi isolated from Antarctica
Gocheva, Yana G.
Tosi, Solveig
Krumova, Ekaterina Tz.
Slokoska, Lyudmila S.
Miteva, Jeny G.
Vassilev, Spassen V.
Angelova, Maria B.
[J]. EXTREMOPHILES, 2009, 13 (02) : 273 - 281

← 1 2 3 4 5 6 →