Frizzled regulates localization of cell-fate determinants and mitotic spindle rotation during asymmetric cell division

被引：198
作者：
Bellaïche, Y
Gho, M
Kaltschmidt, JA
Brand, AH
Schweisguth, F
机构：
[1] Ecole Normale Super, UMR 8544, F-75230 Paris 05, France
[2] Univ Cambridge, Wellcome, CRC Inst, Cambridge CB2 1QR, England
[3] Univ Cambridge, Dept Genet, Cambridge CB2 1QR, England
来源：
NATURE CELL BIOLOGY | 2001年 / 3卷 / 01期
基金：
英国惠康基金;
关键词：
D O I：
10.1038/35050558
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Cell-fate diversity is generated in part by the unequal segregation of cell-fate determinants during asymmetric cell divisions. In the Drosophila pupa, the pI sense organ precursor cell is polarized along the anterior-posterior axis of the fly and divides asymmetrically to generate a posterior pIIa cell and an anterior pIIb cell. The anterior pIIb cell specifically inherits the determinant Numb and the adaptor protein Partner of Numb (Pon). By labelling both the Pon crescent and the microtubules in living pupae, we show that determinants localize at the anterior cortex before mitotic-spindle formation; and that the spindle forms with random orientation and rotates to line up with the Pon crescent. By imaging living frizzled (fz) mutant pupae we show that Fz regulates the orientation of the polarity axis of pI, the initiation of spindle rotation and the unequal partitioning of determinants. We conclude that Fz participates in establishing the polarity of pI.
引用
收藏
页码：50 / 57
页数：8
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