In vivo tumor-targeted dual-modal fluorescence/CT imaging using a nanoprobe co-loaded with an aggregation-induced emission dye and gold nanoparticles

被引：154

作者：

Zhang, Jimei ^{[1
,2
]}

Li, Chan ^{[2
]}

Zhang, Xu ^{[2
]}

Huo, Shuaidong ^{[2
]}

Jin, Shubin ^{[2
]}

An, Fei-Fei ^{[2
]}

Wang, Xiaodan ^{[1
]}

Xue, Xiangdong ^{[2
]}

Okeke, C. I. ^{[2
]}

Duan, Guiyun ^{[1
]}

Guo, Fengguang ^{[1
]}

Zhang, Xiaohong ^{[3
,4
]}

Hao, Jifu ^{[1
]}

Wang, Paul C. ^{[5
]}

Zhang, Jinchao ^{[6
]}

Liang, Xing-Jie ^{[2
]}

机构：

[1] Taishan Med Univ, Coll Pharm, Tai An 271016, Shandong, Peoples R China

[2] Chinese Acad Sci, Key Lab Biol Effects Nanomat & Nanosafety, Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China

[3] Chinese Acad Sci, Tech Inst Phys & Chem, Nanoorgan Photoelect Lab, Beijing 100190, Peoples R China

[4] Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Optoelect Mat, Beijing 100190, Peoples R China

[5] Howard Univ, Dept Radiol, Lab Mol Imaging, Washington, DC 20060 USA

[6] Hebei Univ, Coll Chem & Environm Sci, Chem Biol Key Lab Hebei Prov, Baoding 071002, Peoples R China

来源：

BIOMATERIALS | 2015年 / 42卷

基金：

中国国家自然科学基金;

关键词：

Dual-modal imaging; AIE dye; Gold nanoparticles; Tumor-targeting; Non-invasive fluorescence imaging; X-ray computed tomography; MULTIFUNCTIONAL NANOPARTICLES; VITRO; BIOPROBES; DOTS;

D O I：

10.1016/j.biomaterials.2014.11.053

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

As an intensely studied computed tomography (CT) contrast agent, gold nanoparticle has been suggested to be combined with fluorescence imaging modality to offset the low sensitivity of CT. However, the strong quenching of gold nanoparticle on fluorescent dyes requires complicated design and shielding to overcome. Herein, we report a unique nanoprobe (M-NPAPF-Au) co-loading an aggregation-induced emission (AIE) red dye and gold nanoparticles into DSPE-PEG(2000) micelles for dual-modal fluorescence/CT imaging. The nanoprobe was prepared based on a facile method of "one-pot ultrasonic emulsification". Surprisingly, in the micelles system, fluorescence dye (NPAPF) efficiently overcame the strong fluorescence quenching of shielding-free gold nanoparticles and retained the crucial AIE feature. In vivo studies demonstrated the nanoprobe had superior tumor-targeting ability, excellent fluorescence and CT imaging effects. The totality of present studies clearly indicates the significant potential application of M-NPAPF-Au as a dual-modal non-invasive fluorescence/X-ray CT nanoprobe for in vivo tumor-targeted imaging and diagnosis. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：103 / 111

页数：9