High dose of epigallocatechin-3-gallate inhibits proliferation and induces apoptosis of H9C2 cardiomyocytes through down-regulation of SIRT1

被引:6
作者
Cai, Yi [1 ]
Zhao, Li [1 ]
Qin, Yuan [1 ]
He, Yanhuan [1 ]
机构
[1] Guangzhou Med Univ, Guangzhou Res Inst Snake Venom, Guangzhou 510182, Guangdong, Peoples R China
来源
PHARMAZIE | 2015年 / 70卷 / 01期
基金
中国国家自然科学基金;
关键词
OXIDATIVE STRESS; GREEN TEA; CELLS; EGCG;
D O I
10.1691/ph.2015.4717
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Background: Previous studies have suggested that high doses of (-)-epigallocatechin-3-gallate (EGCG) can induce toxicity in the liver, kidneys, and intestine. However, there have been no reports of myocardiotoxicity following treatment with EGCG. In this study, we investiged the proliferation and apoptosis of H9C2 cardiomyocytes treated with high dose of EGCG. Methods: Cell proliferation was measured by CCK8 assay, cell apoptosis rate was evaluated by TUNEL assay, and the expression alterations of Sirtuin 1 (SIRT1) protein was detected by Western blotting. Results: EGCG inhibits proliferation and induces apoptosis in time- and dose-dependent manner in H9C2 cardiomyocytes. SIRT1 participates in the inhibitory effect of EGCG on cell proliferation and apoptosis induction in H9C2 cardiomyocytes. Conclusion: This study demonstrates that high doses of EGCG inhibit proliferation and induce apoptosis in H9C2 cardiomyocytes. Down-regulation of SIRT 1 protein expression may be involved.
引用
收藏
页码:12 / 16
页数:5
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