Neural cell adhesion molecule - Description of a CSF ELISA method and evidence of reduced levels in selected neurological disorders

被引：27

作者：

Gnanapavan, Sharmilee ^{[1
,2
]}

Grant, Donna ^{[1
]}

Illes-Toth, Eva ^{[1
]}

Lakdawala, Neghat ^{[1
]}

Keir, Geoff ^{[1
]}

Giovannoni, Gavin ^{[2
]}

机构：

[1] Inst Neurol, Dept Neuroimmunol, London WC1N 3BG, England

[2] Barts & London Queen Marys Sch Med & Dent, Ctr Neurosci, London, England

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2010年 / 225卷 / 1-2期

关键词：

Neural cell adhesion molecule; ELISA; Multiple sclerosis; Cerebrospinal fluid; Validation; Nerve regeneration; N-CAM; NERVOUS-SYSTEM; TRANSMEMBRANE DISPOSITION; CEREBROSPINAL-FLUID; SOLUBLE FORMS; NCAM; SERUM; BRAIN; PLASTICITY; ISOFORMS;

D O I：

10.1016/j.jneuroim.2010.04.020

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Neural cell adhesion molecule (NCAM) is important for neuronal growth and repair. Here we describe the development and validation of a sensitive ELISA for NCAM using commercially available reagents. The measurable range of NCAM ELISA is 16-500 ng/mL, with a constant coefficient-of variation and good parallelism between the reference standard curve and CSF. CSF NCAM was measured in 36 benign-intracranial hypertension, 51 multiple sclerosis, 27 neuropathy, 37 Alzheimer's disease, 12 cognitive impairment, 15 motoneurone disease, 13 meningitis, 17 encephalitis, and 17 control cases. Significant reductions were found between controls and multiple sclerosis, Alzheimer's disease and meningitis. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：118 / 122

页数：5

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