The effects of the Chinese medicine ZiBu PiYin recipe on the hippocampus in a rat model of diabetes-associated cognitive decline: a proteomic analysis

被引：76

作者：

Shi, X. ^{[1
]}

Lu, X. G. ^{[2
]}

Zhan, L. B. ^{[1
,3
]}

Qi, X. ^{[4
]}

Liang, L. N. ^{[1
]}

Hu, S. Y. ^{[1
]}

Yan, Y. ^{[1
]}

Zhao, S. Y. ^{[1
]}

Sui, H. ^{[3
]}

Zhang, F. L. ^{[5
]}

机构：

[1] Dalian Med Univ, Affiliated Hosp 2, Dalian 116023, Liaoning Prov, Peoples R China

[2] Dalian Univ, Zhongshan Hosp, Dept Emergency Med, Dalian, Liaoning Prov, Peoples R China

[3] Dalian Med Univ, Inst Integrat Med, Dalian 116023, Liaoning Prov, Peoples R China

[4] Stanford Univ, Sch Med, Dept Chem & Syst Biol, Stanford, CA 94305 USA

[5] Dalian Med Univ, Dept Publ Hlth, Dalian 116023, Liaoning Prov, Peoples R China

来源：

DIABETOLOGIA | 2011年 / 54卷 / 07期

关键词：

Chinese medicine ZiBu PiYin recipe; Diabetes-associated cognitive decline; DIGE; MALDI-TOF/TOF mass spectrometry; Proteomics; Type; 2; diabetes; STREPTOZOTOCIN-TREATED RAT; SYNAPTIC PLASTICITY; ALZHEIMERS-DISEASE; BRAIN; MELLITUS; GLUTATHIONE; INSULIN; ENCEPHALOPATHY; PERFORMANCE; EXPRESSION;

D O I：

10.1007/s00125-011-2147-z

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Increasing evidence suggests that diabetes is associated with an enhanced risk of cognitive decline. The precise mechanisms underlying diabetes-associated cognitive decline (DACD) remain unclear. Here we investigated the molecular changes associated with DACD using a comparative proteomics study of hippocampus in a rat model of type 2 diabetes. In addition, we tested the effects of the Chinese medicine ZiBu PiYin recipe (ZBPYR) on DACD. The hippocampus was dissected from control, diabetic and diabetic rats treated with ZBPYR (DM/ZBPYR). Soluble proteins were separated using fluorescence-based difference gel electrophoresis. Protein spots were visualised with fluorescent dyes and spot density was compared between each pair of groups. Proteins of interest were identified using mass spectrometry. Proteins of specific interest were also tested by western blot and real-time PCR analysis. We found 13 spots that were altered between control and diabetes groups, and 12 spots that were changed between diabetes and DM/ZBPYR groups. The identities of nine proteins were determined by mass spectrometry. The identified proteins were largely involved in energy metabolism, cytoskeleton regulation and oxidative stress. The protein alterations observed in the diabetes group were ameliorated to varying degrees following ZBPYR treatment. The protein changes identified in hippocampus from a rat model of type 2 diabetes suggest that specific cellular alterations contribute to DACD. The Chinese medicine ZBPYR was found to affect multiple targets and partially repaired the original cellular balance. This study may provide important insights into the molecular events underlying DACD and allow the identification of novel therapeutic targets.

引用

页码：1888 / 1899

页数：12

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