EFFECTS OF GAMMA RAY ON SOME MOLECULAR CHARACTERISTICS OF IN VITRO REGENERATED GERBERA JAMESONII BOLUS

被引：0
|
作者：
Akter, Nazma [1 ]
Sarker, R. H. [1 ]
Hoque, M. I. [1 ]
机构：
[1] Univ Dhaka, Plant Breeding & Biotechnol Lab, Dept Bot, Dhaka 1000, Bangladesh
来源：
BANGLADESH JOURNAL OF BOTANY | 2010年 / 39卷 / 02期
关键词：
Gerbera; In vitro regeneration; Gamma radiation; PAGE; Isozyme studies; CHRYSANTHEMUM; IRRADIATION;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Gamma radiation (Co-60) of 20 and 15 Gy at 6.5 Gy/min applied on callus with shoot primordia, single shoot and multiple shoots of Gerbera jamesonii Bolus revealed that 15 Gy was more effective in the induction, of mutation. Survival percentage after irradiation was highest in cultures with multiple shoots. Isozyme studies showed the presence Of different banding profiles following polyacrylamide gel electrophoresis. The resulted bands have been found to be different in respect of their intensity, number and pattern. Such isozyme studies can be reliably used as markers in characterizing induced variation.
引用
收藏
页码：207 / 213
页数：7
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