Effects of Substance Use and Sex Practices on the Intestinal Microbiome During HIV-1 Infection

被引:38
作者
Fulcher, Jennifer A. [1 ,4 ]
Hussain, Shehnaz K. [5 ,6 ]
Cook, Ryan [6 ]
Li, Fan [2 ]
Tobin, Nicole H. [2 ]
Ragsdale, Amy
Shoptaw, Steven [3 ]
Gorbach, Pamina M. [1 ,6 ]
Aldrovandi, Grace M. [2 ]
机构
[1] Univ Calif Los Angeles, David Geffen Sch Med, Dept Med, Div Infect Dis, Los Angeles, CA 90024 USA
[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pediat, Div Infect Dis, Los Angeles, CA 90024 USA
[3] Univ Calif Los Angeles, David Geffen Sch Med, Dept Family Med, Los Angeles, CA 90024 USA
[4] Univ Calif Los Angeles, VA Greater Los Angeles Healthcare Syst, Los Angeles, CA USA
[5] Univ Calif Los Angeles, Cedars Sinai Med Ctr, Dept Med, Los Angeles, CA USA
[6] Univ Calif Los Angeles, Fielding Sch Publ Hlth, Dept Epidemiol, Los Angeles, CA USA
基金
美国国家卫生研究院;
关键词
Microbiome; substance use; HIV-1; infection; men who have sex with men; INFLAMMATION; CANNABINOIDS; MODELS; DAMAGE;
D O I
10.1093/infdis/jiy349
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. Human immunodeficiency virus type 1 (HIV-1) infection alters the human intestinal microbiome; however, behavioral factors driving these changes remain poorly defined. Here we examine the effects of substance use and sex behavior on the microbiome during HIV-1 infection. Methods. Archival rectal swab specimens, urine drug test results, and responses to substance use and sex behavior questionnaires were obtained from 37 HIV-positive participants at 2 time points, separated by 6 months, in a cohort examining the effects of substance use in men who have sex with men (MSM). Microbiome profiling was performed using 16S ribosomal RNA gene sequencing, and associations with behavioral factors were examined using 0-inflated negative binomial regression. Further analysis of selected variables of interest was performed using propensity scores to account for multiple confounders. Results. Using permutational multivariate analysis of variance, we found that receptive anal intercourse, methamphetamine use, and marijuana use were among the most important drivers of microbiome variation. Propensity score-adjusted analyses revealed that methamphetamine use and marijuana use displayed unique associations; methamphetamine use was associated with an increased abundance of Porphyromonas and Granulicatella organisms and a decreased abundance of Ruminococcus, Collinsella, and Parabacteroides organisms, whereas marijuana use was associated with an increased abundance of Ruminococcus, Clostridium cluster IV, Solobacterium, and Fusobacterium organisms and a decreased abundance of Acidaminococcus, Prevotella, Dialister, Anaerostipes, and Dorea organisms. Conclusions. Drug use and sex behavior are important factors associated with intestinal dysbiosis during chronic HIV-1 infection among young MSM.
引用
收藏
页码:1560 / 1570
页数:11
相关论文
共 42 条
[1]  
[Anonymous], 2016, TOOLKIT WEIGHTING AN
[2]   Oral pathobiont induces systemic inflammation and metabolic changes associated with alteration of gut microbiota [J].
Arimatsu, Kei ;
Yamada, Hitomi ;
Miyazawa, Haruna ;
Minagawa, Takayoshi ;
Nakajima, Mayuka ;
Ryder, Mark I. ;
Gotoh, Kazuyoshi ;
Motooka, Daisuke ;
Nakamura, Shota ;
Iida, Tetsuya ;
Yamazaki, Kazuhisa .
SCIENTIFIC REPORTS, 2014, 4
[3]   Opioid-induced gut microbial disruption and bile dysregulation leads to gut barrier compromise and sustained systemic inflammation [J].
Banerjee, S. ;
Sindberg, G. ;
Wang, F. ;
Meng, J. ;
Sharma, U. ;
Zhang, L. ;
Dauer, P. ;
Chen, C. ;
Dalluge, J. ;
Johnson, T. ;
Roy, S. .
MUCOSAL IMMUNOLOGY, 2016, 9 (06) :1418-1428
[4]  
Begay O, 2011, AIDS RES HUM RETROV, V27, P1019, DOI [10.1089/aid.2010.0252, 10.1089/AID.2010.0252]
[5]   Role of the Microbiota in Immunity and Inflammation [J].
Belkaid, Yasmine ;
Hand, Timothy W. .
CELL, 2014, 157 (01) :121-141
[6]   Maternal HIV infection influences the microbiome of HIV-uninfected infants [J].
Bender, Jeffrey M. ;
Li, Fan ;
Martelly, Shoria ;
Byrt, Erin ;
Rouzier, Vanessa ;
Leo, Marguerite ;
Tobin, Nicole ;
Pannaraj, Pia S. ;
Adisetiyo, Helty ;
Rollie, Adrienne ;
Santiskulvong, Chintda ;
Wang, Shaun ;
Autran, Chloe ;
Bode, Lars ;
Fitzgerald, Daniel ;
Kuhn, Louise ;
Aldrovandi, Grace M. .
SCIENCE TRANSLATIONAL MEDICINE, 2016, 8 (349)
[7]   Regulation of inflammation by microbiota interactions with the host [J].
Blander, J. Magarian ;
Longman, Randy S. ;
Iliev, Iliyan D. ;
Sonnenberg, Gregory F. ;
Artis, David .
NATURE IMMUNOLOGY, 2017, 18 (08) :851-860
[8]   A role for seminal plasma in modulating pregnancy outcomes in domestic species [J].
Bromfield, John J. .
REPRODUCTION, 2016, 152 (06) :R223-R232
[9]  
Callahan BJ, 2016, NAT METHODS, V13, P581, DOI [10.1038/NMETH.3869, 10.1038/nmeth.3869]
[10]   Endocannabinoids - at the crossroads between the gut microbiota and host metabolism [J].
Cani, Patrice D. ;
Plovier, Hubert ;
Van Hul, Matthias ;
Geurts, Lucie ;
Delzenne, Nathalie M. ;
Druart, Celine ;
Everard, Amandine .
NATURE REVIEWS ENDOCRINOLOGY, 2016, 12 (03) :133-143