One- and two-photon time-resolved fluorescence study of neurotransmitter amino acid-5,6-benzocoumarin conjugates

被引：10

作者：

Hungerford, Graham ^{[1
]}

Ryderfors, Linus ^{[2
]}

Fernandes, Maria Jose G. ^{[3
]}

Goncalves, M. Sameiro T. ^{[3
]}

Costa, Susana P. G. ^{[3
]}

机构：

[1] HORIBA Jobin Yvon IBH Ltd, Glasgow G3 8JU, Lanark, Scotland

[2] Univ Strathclyde, Dept Phys, Glasgow G4 0NG, Lanark, Scotland

[3] Univ Minho, Ctr Chem, P-4710057 Braga, Portugal

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY | 2010年 / 215卷 / 2-3期

关键词：

Amino acids; Benzocoumarin; Time-resolved emission spectrum; Two-photon excitation; EXCITATION CROSS-SECTIONS; SINGLE-PHOTON; PHOTOCLEAVAGE; SPECTROSCOPY; DERIVATIVES; MECHANISM; PROBES;

D O I：

10.1016/j.jphotochem.2010.08.020

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The time-resolved photophysical characterisation of a set of fluorescent conjugates of neurotransmitter amino acids (glutamic acid, tyrosine and beta-alanine) based on a methoxylated 5,6-benzocoumarin fluorophore was performed Both one- and two-photon excitation were employed to excite the fluorophore and the cross-section with wavelength for the two-photon process determined The photophysical characterisation was supported using a molecular modelling study, which helped elucidate the molecular transitions (by a ZINDO calculation) and differences in the electron density between the HOMO and LUMO in geometry (PM3) optimised structures The effect of prolonged irradiation of the conjugates was monitored via fluorimetry and differences in the kinetics of the process observed were found to depend on the amino acid employed. (C) 2010 Elsevier B.V. All rights reserved

引用

页码：214 / 222

页数：9

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