Cell viability, reactive oxygen species, apoptosis, and necrosis in myoblast cultures exposed to low-level infrared laser

被引:24
作者
da Silva Neto Trajano, Larissa Alexsandra [1 ,2 ]
da Silva, Camila Luna [1 ]
de Carvalho, Simone Nunes [1 ]
Cortez, Erika [1 ]
Mencalha, Andre Luiz [2 ]
da Fonseca, Adenilson de Souza [2 ,3 ,4 ]
Stumbo, Ana Carolina [1 ]
机构
[1] Univ Estado Rio de Janeiro, Inst Biol Roberto Alcantara Gomes, Lab Pesquisa Celulas Tronco, Dept Histol & Embriol,PAPC, Ave Prof Manuel de Abreu 444,3 Andar, BR-20551170 Rio De Janeiro, Brazil
[2] Univ Estado Rio de Janeiro, Inst Biol Roberto Alcantara Gomes, Dept Biofis & Biometria, Ave 28 Setembro,87 Fundos, BR-20551030 Rio De Janeiro, Brazil
[3] Univ Fed Estado Rio de Janeiro, Inst Biomed, Dept Ciencias Fisiol, Rua Frei Caneca 94, BR-20211040 Rio De Janeiro, Brazil
[4] Univ Estado Rio de Janeiro, Inst Biol Roberto Alcantara Gomes, Dept Biofis & Biometria, Rua Sao Francisco Xavier 524, BR-20550900 Rio De Janeiro, Brazil
关键词
Apoptosis; Cell viability; Reactive oxygen species; Low-level laser therapy; Myoblast; Necrosis; MEDIATED PHOTODYNAMIC THERAPY; SKELETAL-MUSCLE; IN-VITRO; ENERGY; PROLIFERATION; REPAIR;
D O I
10.1007/s10103-016-1909-8
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Low-level infrared laser is considered safe and effective for treatment of muscle injuries. However, the mechanism involved on beneficial effects of laser therapy are not understood. The aim was to evaluate cell viability, reactive oxygen species, apoptosis, and necrosis in myoblast cultures exposed to low-level infrared laser at therapeutic fluences. C2C12 myoblast cultures at different (2 and 10 %) fetal bovine serum (FBS) concentrations were exposed to low-level infrared laser (808 nm, 100 mW) at different fluences (10, 35, and 70 J/cm(2)) and evaluated after 24, 48, and 72 h. Cell viability was evaluated by WST-1 assay; reactive oxygen species (ROS), apoptosis, and necrosis were evaluated by flow cytometry. Cell viability was decreased atthe lowest FBS concentration. Laser exposure increased the cell viability in myoblast cultures at 2 % FBS after 48 and 72 h, but no significant increase in ROS was observed. Apoptosis was decreased at the higher fluence and necrosis was increased at lower fluence in myoblast cultures after 24 h of laser exposure at 2 % FBS. No laser-induced alterations were obtained at 10 % FBS. Results show that level of reactive oxygen species is not altered, at least to those evaluated in this study, but low-level infrared laser exposure affects cell viability, apoptosis, and necrosis in myoblast cultures depending on laser fluence and physiologic conditions of cells.
引用
收藏
页码:841 / 848
页数:8
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