Genes determining yeast replicative life span in a long-lived genetic background

被引:129
作者
Kaeberlein, M
Kirkland, KT
Fields, S
Kennedy, BK [1 ]
机构
[1] Univ Washington, Dept Biochem, Seattle, WA 98195 USA
[2] Univ Washington, Dept Genome Sci & Med, Seattle, WA 98195 USA
[3] Univ Washington, Howard Hughes Med Inst, Seattle, WA 98195 USA
关键词
yeast aging; caloric restriction; genetic pathways;
D O I
10.1016/j.mad.2004.10.007
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Here we describe the replicative life spans of more than 50 congenic Saccharomyces cerevisiae strains, each carrying a mutation previously implicated in yeast aging. This analysis provides a direct comparison, in a single, long-lived strain background, of a majority of reported yeast aging genes. Of the eleven deletion mutations previously reported to increase yeast life span, we find that deletion of FOB1, deletion of SCH9, and deletion of GPA2, GPR1, or HXK2 (three genetic models of calorie restriction) significantly enhanced longevity. In addition, overexpression of SIR2 or growth on low glucose increased life span. These results define a limited number of genes likely to regulate replicative life span in a strain-independent manner, and create a basis for future epistasis analysis to determine genetic pathways of aging. (c) 2004 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:491 / 504
页数:14
相关论文
共 64 条
[61]   Deficiency in superoxide dismutases shortens life span of yeast cells [J].
Wawryn, J ;
Krzepilko, A ;
Myszka, A ;
Bilinski, T .
ACTA BIOCHIMICA POLONICA, 1999, 46 (02) :249-253
[62]   Effect of superoxide dismutase deficiency on the life span of the yeast Saccharomyces cervisiae.: An oxygen-independent role of Cu,Zn-superoxide dismutase [J].
Wawryn, J ;
Swiecilo, A ;
Bartosz, G ;
Bilinski, T .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2002, 1570 (03) :199-202
[63]   A Saccharomyces cerevisiae mutant with increased virulence [J].
Wheeler, RT ;
Kupiec, M ;
Magnelli, P ;
Abeijon, C ;
Fink, GR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (05) :2766-2770
[64]   Functional characterization of the S-cerevisiae genome by gene deletion and parallel analysis [J].
Winzeler, EA ;
Shoemaker, DD ;
Astromoff, A ;
Liang, H ;
Anderson, K ;
Andre, B ;
Bangham, R ;
Benito, R ;
Boeke, JD ;
Bussey, H ;
Chu, AM ;
Connelly, C ;
Davis, K ;
Dietrich, F ;
Dow, SW ;
EL Bakkoury, M ;
Foury, F ;
Friend, SH ;
Gentalen, E ;
Giaever, G ;
Hegemann, JH ;
Jones, T ;
Laub, M ;
Liao, H ;
Liebundguth, N ;
Lockhart, DJ ;
Lucau-Danila, A ;
Lussier, M ;
M'Rabet, N ;
Menard, P ;
Mittmann, M ;
Pai, C ;
Rebischung, C ;
Revuelta, JL ;
Riles, L ;
Roberts, CJ ;
Ross-MacDonald, P ;
Scherens, B ;
Snyder, M ;
Sookhai-Mahadeo, S ;
Storms, RK ;
Véronneau, S ;
Voet, M ;
Volckaert, G ;
Ward, TR ;
Wysocki, R ;
Yen, GS ;
Yu, KX ;
Zimmermann, K ;
Philippsen, P .
SCIENCE, 1999, 285 (5429) :901-906