Two-photon excited fluorescence lifetime imaging microscopy for FRET study on protein interactions

被引：1

作者：

Qu, JL ^{[1
]}

Lin, ZY ^{[1
]}

Liu, LX ^{[1
]}

Guo, X ^{[1
]}

Chen, DN ^{[1
]}

Niu, HB ^{[1
]}

机构：

[1] Shenzhen Univ, Inst Optoelect, Shenzhen 518060, Peoples R China

来源：

OPTICS IN HEALTH CARE AND BIOMEDICAL OPTICS: DIAGNOSTICS AND TREATMENT II , PTS 1 AND 2 | 2005年 / 5630卷

关键词：

fluorescence lifetime imaging; fluorescence resonance energy transfer; time-correlated single photon counting; two-photon excited fluorescence;

D O I：

10.1117/12.569582

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Two-photon excited fluorescence lifetime imaging (2P-FLIM) provides a more direct and precise approach to fluorescence resonance energy transfer (FRET), which allows studying the dynamic behavior of protein-protein interactions in living cells. In this paper, we describe the combination of a Leica TCS SP2 laser scanning microscope and a time-correlated single photon counting (TCSPC) lifetime imaging module developed by Becker & Hick] for two-photon excited fluorescence lifetime imaging. This 2P-FLIM system was used for FRET study on the interaction of heat shock protein hsp27 with p38 MAP kinase in the single living cell. Results show that the reduction in donor (CFP) lifetime in the presence of acceptor (YFP) reveals interactions between the two proteins.

引用

页码：517 / 522

页数：6

共 50 条

[1] Optogenetic Imaging of Protein Activity Using Two-Photon Fluorescence Lifetime Imaging Microscopy
Murakoshi, Hideji
OPTOGENETICS: LIGHT-SENSING PROTEINS AND THEIR APPLICATIONS IN NEUROSCIENCE AND BEYOND, 2ND EDITION, 2021, 1293 : 295 - 308
[2] Characterization of two-photon excitation fluorescence lifetime imaging microscopy for protein localization
Chen, YE
Periasamy, A
MICROSCOPY RESEARCH AND TECHNIQUE, 2004, 63 (01) : 72 - 80
[3] Two-photon excited fluorescence lifetime ophhalmoscopy
Hunter, Jennifer J.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2023, 64 (08)
[4] Towards two-photon excited endogenous fluorescence lifetime imaging microendoscopy
Hage, C. H.
Leclerc, P.
Brevier, J.
Fabert, M.
Le Nezet, C.
Kudlinski, A.
Heliot, L.
Louradour, F.
BIOMEDICAL OPTICS EXPRESS, 2018, 9 (01): : 142 - 156
[5] Experimental study on multicolor two-photon excited fluorescence microscopy
Qiu Jun-Peng
Liang Run-Fu
Peng Xiao
Li Ya-Hui
Liu Li-Xin
Yin Jun
Qu Jun-Le
Niu Han-Ben
ACTA PHYSICA SINICA, 2015, 64 (04)
[6] Metabolic Imaging Using Two-Photon Excited NADH Intensity and Fluorescence Lifetime Imaging
Vergen, Jorge
Hecht, Clifford
Zholudeva, Lyandysha V.
Marquardt, Meg M.
Hallworth, Richard
Nichols, Michael G.
MICROSCOPY AND MICROANALYSIS, 2012, 18 (04) : 761 - 770
[7] Mapping of Intracellular Concentrations of Macromolecules by Two-photon Excited Fluorescence Lifetime Imaging
Liu, Lixin
Pliss, Artem
Peng, Xiao
Kuzmin, Andrey
Qu, Junle
Prasad, Paras N.
MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XVI, 2016, 9712
[8] Two-photon excited fluorescence lifetime imaging and spectroscopy of melanins in vitro and in vivo
Krasieva, Tatiana B.
Stringari, Chiara
Liu, Feng
Sun, Chung-Ho
Kong, Yu
Balu, Mihaela
Meyskens, Frank L.
Gratton, Enrico
Tromberg, Bruce J.
JOURNAL OF BIOMEDICAL OPTICS, 2013, 18 (03)
[9] Two-photon excitation in fluorescence lifetime imaging
Gerritsen, HC
Vroom, J
Sytsma, J
FLUORESCENCE MICROSCOPY AND FLUORESCENT PROBES, VOL 2, 1998, : 55 - 62
[10] Monitor RNA synthesis in live cell nuclei by using two-photon excited fluorescence lifetime imaging microscopy
Peng, Xiao
Lin, Danying
Wang, Yan
Qi, Jing
Yan, Wei
Qu, Junle
IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES XIII, 2015, 9328

← 1 2 3 4 5 →