Organic acid cross-linked 3D printed cellulose nanocomposite bioscaffolds with controlled porosity, mechanical strength, and biocompatibility

被引：23

作者：

Stiglic, Andreja Dobaj ^{[1
]}

Gurer, Fazilet ^{[1
]}

Lackner, Florian ^{[2
,10
]}

Bracic, Doris ^{[1
]}

Winter, Armin ^{[3
]}

Gradisnik, Lidija ^{[4
]}

Makuc, Damjan ^{[5
]}

Kargl, Rupert ^{[1
,2
,10
]}

Duarte, Isabel ^{[6
]}

Plavec, Janez ^{[5
,7
,8
]}

Maver, Uros ^{[4
]}

Beaumont, Marco ^{[9
]}

Kleinschek, Karin Stana ^{[2
,10
]}

Mohan, Tamilselvan ^{[1
,2
,10
]}

机构：

[1] Univ Maribor, Lab Characterizat & Proc Polymers, Fac Mech Engn, Smetanova Ulica 17, Maribor 2000, Slovenia

[2] Graz Univ Technol, Inst Chem & Technol Biobased Syst IBioSys, Stremayrgasse 9, A-8010 Graz, Austria

[3] Univ Nat Resources & Life Sci, BOKU, Inst Wood Technol & Renewable Mat, Konrad Lorenz Str 24, A-3430 Tulln, Austria

[4] Univ Maribor, Inst Biomed Sci, Fac Med, Taborska Ulica 8, Maribor 2000, Slovenia

[5] Slovenian NMR Ctr, Natl Inst Chem, Hajdrihova 19, Ljubljana 1001, Slovenia

[6] Univ Aveiro, Dept Mech Engn, Ctr Mech Technol & Automat, Campus Univ Santiago, P-3810193 Aveiro, Portugal

[7] EN FIST Ctr Excellence, Trg 13, Ljubljana 1000, Slovenia

[8] Univ Ljubljana, Fac Chem & Chem Technol, Vecna pot 113, Ljubljana 1000, Slovenia

[9] Univ Nat Resources & Life Sci BOKU, Inst Chem Renewable Resources, Konrad Lorenz Str 24, A-3430 Tulln, Austria

[10] European Polysaccharide Network Excellence EPNOE, Leuven, Belgium

来源：

ISCIENCE | 2022年 / 25卷 / 05期

关键词：

POROUS SCAFFOLD; LINKING; BONE; HYDROGELS; RELEASE; FILMS;

D O I：

10.1016/j.isci.2022.104263

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Herein, we fabricated chemically cross-linked polysaccharide-based three-dimensional ( 3D) porous scaffolds using an ink composed of nanofibrillated cellulose, carboxymethyl cellulose, and citric acid (CA), featuring strong shear thinning behavior and adequate printability. Scaffolds were produced by combining direct-ink-writing 3D printing, freeze-drying, and dehydrothermal heat-assisted cross-linking techniques. The last step induces a reaction of CA. Degree of crosslinking was controlled by varying the CA concentration (2.5-10.0 wt.%) to tune the structure, swelling, degradation, and surface properties (pores: 100-450 mm, porosity: 86%) of the scaffolds in the dry and hydrated states. Compressive strength, elastic modulus, and shape recovery of the cross-linked scaffolds increased significantly with increasing cross-linker concentration. Cross-linked scaffolds promoted clustered cell adhesion and showed no cytotoxic effects as determined by the viability assay and live/dead staining with human osteoblast cells. The proposed method can be extended to all polysaccharide-based materials to develop cell-friendly scaffolds suitable for tissue engineering applications.

引用

页数：23

共 59 条

[31] Nanocellulose-enriched hydrocolloid-based hydrogels designed using a Ca2+ free strategy based on citric acid [J].