Photocycle of 13-cis-retinal in bacteriorhodopsin caused by destruction to the cooperative effect of purple membrane

被引：1
|
作者：
Hu, KS [1 ]
He, JA [1 ]
Zhang, HT [1 ]
机构：
[1] Chinese Acad Sci, Inst Biophys, Dept Cell Biol, Beijing 100101, Peoples R China
来源：
SCIENCE IN CHINA SERIES B-CHEMISTRY | 1998年 / 41卷 / 02期
关键词：
bacteriorhodopsin; purple membrane; cooperative effect; Triton X-100; M-412; intermediate;
D O I：
10.1007/BF02877798
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The steady absorption and kinetic changes of M-412 intermediate of the light-and dark-adapted bacteriorhodopsin (BR) solubilized by different concentrated Triton X-100 were investigated. The results indicated that the cooperative effect existing within the trimeric BR of native purple membrane (PM) was damaged in the system containing the surfactant since the component and structure of the bilayer lipid membrane in PM varied due to the solubilization of partial PM lipids by Triton X-100. The destruction to the cooperative effect of BR ultimately caused 13-cis-retinal of the dark-adapted BR to take part in BR photocycle and also to generate the deprotonated M-412 intermediate.
引用
收藏
页码：135 / 141
页数：7
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