Preparation and biocompatibility of nanohybrid scaffolds by in situ homogeneous formation of nano hydroxyapatite from biopolymer polyelectrolyte complex for bone repair applications

被引：50
作者：
Chen, Jingdi [1 ]
Yu, Qifeng [1 ,2 ]
Zhang, Guodong [1 ,2 ]
Yang, Shen [1 ,2 ]
Wu, Jiulin [1 ]
Zhang, Qiqing [1 ,3 ,4 ]
机构：
[1] Fuzhou Univ, Inst Biomed & Pharmaceut Technol, Fuzhou 350002, Peoples R China
[2] Fuzhou Univ, Coll Chem & Chem Engn, Fuzhou 350108, Peoples R China
[3] Chinese Acad Med Sci, Inst Biomed Engn, Tianjin 300192, Peoples R China
[4] Peking Union Med Coll, Tianjin 300192, Peoples R China
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2012年 / 93卷
基金：
中国国家自然科学基金;
关键词：
Chitosan; Hyaluronic acid; Homogeneous; Nano hydroxyapatite; Scaffold; MESENCHYMAL STEM-CELLS; HYALURONIC-ACID; CARBON NANOTUBES; CHITOSAN; MINERALIZATION; PHOSPHATE; HYDROGELS; BEHAVIOR; NETWORK; FILMS;
D O I：
10.1016/j.colsurfb.2011.12.022
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
The achievement of nano distribution for inorganic reinforced filler is a big challenge to three-dimensional porous composite scaffolds. In this paper, a homogeneous nano hydroxyapatite/polyelectrolyte complex (HAP/PEC) hybrid scaffold was developed and investigated. Based on the enhancing properties of the formation of PEC between chitosan and hyaluronic acid, the introduction of nano HAP via in situ crystallization from the PEC achieved nano distribution in the PEC matrix and supplied nano topographies of extracellular environments for the nanohybrid scaffold. The biocompatibility and bioactivity were evaluated by Human bone mesenchymal stem cells (hBMSCs) proliferation (MTT assay), maturation (alkaline phosphatase (ALP) activity) and histological analysis. The in vitro tests show the scaffold is excellent for cell penetration, growth, and proliferation and it is promising for bone repair application. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：100 / 107
页数：8
相关论文
共 38 条
[1]   Self-organization of hydroxyapatite nanorods through oriented attachment [J].
Chen, Jing Di ;
Wang, Ying Jun ;
Wei, Kun ;
Zhang, Shu Hua ;
Shi, Xue Tao .
BIOMATERIALS, 2007, 28 (14) :2275-2280
[2]   In Situ Fabrication of Nano-hydroxyapatite in a Macroporous Chitosan Scaffold for Tissue Engineering [J].
Chen, Jing Di ;
Wang, Yingjun ;
Chen, Xiaofeng .
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2009, 20 (11) :1555-1565
[3]   A simple sol-gel technique for synthesis of nanostructured hydroxyapatite, tricalcium phosphate and biphasic powders [J].
Chen, Jingdi ;
Wang, Yingjun ;
Chen, Xiaofeng ;
Ren, Li ;
Lai, Chen ;
He, Wen ;
Zhang, Qiqing .
MATERIALS LETTERS, 2011, 65 (12) :1923-1926
[4]   Characterization and biocompatibility of nanohybrid scaffold prepared via in situ crystallization of hydroxyapatite in chitosan matrix [J].
Chen, Jingdi ;
Nan, Kaihui ;
Yin, Shiheng ;
Wang, Yingjun ;
Wu, Tao ;
Zhang, Qiqing .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2010, 81 (02) :640-647
[5]   Physico/chemical characterization and in vivo evaluation of nanothickness bioceramic depositions on alumina-blasted/acid-etched Ti-6Al-4V implant surfaces [J].
Coelho, Paulo G. ;
Lemons, Jack E. .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2009, 90A (02) :351-361
[6]   Chitosan-chondroitin sulfate and chitosan-hyaluronate polyelectrolyte complexes. Physico-chemical aspects [J].
Denuziere, A ;
Ferrier, D ;
Domard, A .
CARBOHYDRATE POLYMERS, 1996, 29 (04) :317-323
[7]   Amorphous calcium (ortho)phosphates [J].
Dorozhkin, Sergey V. .
ACTA BIOMATERIALIA, 2010, 6 (12) :4457-4475
[8]   Bioceramics of calcium orthophosphates [J].
Dorozhkin, Sergey V. .
BIOMATERIALS, 2010, 31 (07) :1465-1485
[9]   Tissue engineering - Current challenges and expanding opportunities [J].
Griffith, LG ;
Naughton, G .
SCIENCE, 2002, 295 (5557) :1009-+
[10]   Control of in vitro tissue-engineered bone-like structures using human mesenchymal stem cells and porous silk scaffolds [J].
Hofmann, Sandra ;
Hagenmueller, Henri ;
Koch, Annette M. ;
Mueller, Ralph ;
Vunjak-Novakovic, Gordana ;
Kaplan, David L. ;
Merkle, Hans P. ;
Meinel, Lorenz .
BIOMATERIALS, 2007, 28 (06) :1152-1162
1234