High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins

被引：47

作者：

Zawadzka-Kazimierczuk, Anna ^{[1
]}

Kozminski, Wiktor ^{[1
]}

Sanderova, Hana ^{[2
]}

Krasny, Libor ^{[2
]}

机构：

[1] Univ Warsaw, Fac Chem, PL-02093 Warsaw, Poland

[2] Acad Sci Czech Republic, Inst Microbiol, Dept Bacteriol, Lab Mol Genet Bacteria, CR-14220 Prague, Czech Republic

来源：

JOURNAL OF BIOMOLECULAR NMR | 2012年 / 52卷 / 04期

关键词：

Intrinsically disordered proteins; Non-uniform sampling; Backbone assignment; 5D NMR; 6D NMR; MULTIDIMENSIONAL NMR; SPECTROSCOPY; AMIDE;

D O I：

10.1007/s10858-012-9613-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using delta subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences.

引用

页码：329 / 337

页数：9

共 28 条

[1]

[Anonymous], 2002, SPARKY 3

[2] 13C-detected protonless NMR spectroscopy of proteins in solution [J].