A stable microtubule array drives fission yeast polarity reestablishment upon quiescence exit

被引:14
作者
Laporte, Damien [1 ,2 ]
Courtout, Fabien [1 ,2 ]
Pinson, Benoit [1 ,2 ]
Dompierre, Jim [1 ,2 ]
Salin, Benedicte [1 ,2 ]
Brocard, Lysiane [3 ]
Sagot, Isabelle [1 ,2 ]
机构
[1] Univ Bordeaux, Inst Biochim & Genet Cellulaires, F-33000 Bordeaux, France
[2] Ctr Natl Rech Sci, Bordeaux UMR5095, F-33077 Bordeaux, France
[3] INRA, Bordeaux Imaging Ctr, Pole Imagerie Vegetal, F-33140 Villenave Dornon, France
关键词
GAMMA-TUBULIN COMPLEX; INTERPHASE MICROTUBULES; ORGANIZING CENTERS; CELL-DIVISION; OVERLAPPING MICROTUBULES; DYNAMIC INSTABILITY; SELF-ORGANIZATION; NUCLEATION; PROTEINS; MAINTENANCE;
D O I
10.1083/jcb.201502025
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Cells perpetually face the decision to proliferate or to stay quiescent. Here we show that upon quiescence establishment, Schizosaccharomyces pombe cells drastically rearrange both their actin and microtubule (MT) cytoskeletons and lose their polarity. Indeed, while polarity markers are lost from cell extremities, actin patches and cables are reorganized into actin bodies, which are stable actin filament containing structures. Astonishingly, MTs are also stabilized and rearranged into a novel antiparallel bundle associated with the spindle pole body, named Q-MT bundle. We have identified proteins involved in this process and propose a molecular model for Q-MT bundle formation. Finally and importantly, we reveal that Q-MT bundle elongation is involved in polarity reestablishment upon quiescence exit and thereby the efficient return to the proliferative state. Our work demonstrates that quiescent S. pombe cells assemble specific cytoskeleton structures that improve the swiftness of the transition back to proliferation.
引用
收藏
页码:99 / 113
页数:15
相关论文
共 86 条
[21]   Dynamic positioning of the fission yeast cell division plane [J].
Daga, RR ;
Chang, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (23) :8228-8232
[22]   Proliferation/Quiescence: When to start? Where to stop? What to stock? [J].
Daignan-Fornier, Bertrand ;
Sagot, Isabelle .
CELL DIVISION, 2011, 6
[23]   Proliferation/quiescence: the controversial "aller-retour" [J].
Daignan-Fornier, Bertrand ;
Sagot, Isabelle .
CELL DIVISION, 2011, 6
[24]   3-DIMENSIONAL RECONSTRUCTION AND ANALYSIS OF MITOTIC SPINDLES FROM THE YEAST, SCHIZOSACCHAROMYCES-POMBE [J].
DING, R ;
MCDONALD, KL ;
MCINTOSH, JR .
JOURNAL OF CELL BIOLOGY, 1993, 120 (01) :141-151
[25]   Dynamics of interphase microtubules in Schizosaccharomyces pombe [J].
Drummond, DR ;
Cross, RA .
CURRENT BIOLOGY, 2000, 10 (13) :766-775
[26]   S. pombe Kinesins-8 Promote Both Nucleation and Catastrophe of Microtubules [J].
Erent, Muriel ;
Drummond, Douglas R. ;
Cross, Robert A. .
PLOS ONE, 2012, 7 (02)
[27]   Proteome analysis and morphological studies reveal multiple effects of the immunosuppressive drug mycophenolic acid specifically resulting from guanylic nucleotide depletion [J].
Escobar-Henriques, M ;
Balguerie, A ;
Monribot, C ;
Boucherie, H ;
Daignan-Fornier, B .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (49) :46237-46242
[28]   Fission yeast: in shape to divide [J].
Hachet, Olivier ;
Bendezu, Felipe O. ;
Martin, Sophie G. .
CURRENT OPINION IN CELL BIOLOGY, 2012, 24 (06) :858-864
[29]  
Hagan IM, 1998, J CELL SCI, V111, P1603
[30]  
Hagan IM, 2000, CURR TOP DEV BIOL, V49, P133